3. The volume of big square of hemacytometer is 104 ml. Derive the volume (mL) of small square in the central square as shown in below. Depth - 0.100 mm Volume of big square = 104ml Volume of small square =?_ ml Source: https://laboratoryinfo.com/manual-cell-counting-neubauer-chamber/
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- What are two properties or characteristics of a cell or particle that can be measured by a flow cytometer? Please answer with 5 dot points/sentences minimumIn one square of a hemacytometer you count 55 cells. What is the approximate concentration of cells per l (liter) in this sample? (note that "^" means superscript, e.g. 10^2 means 10 to the 2nd power) The options are 5.5 X 10^5 2.75 X 10^8 5.5 X 10^8 2.75 X 10^56.1 EXPECTED RESULT FOR CELL COUNT USING HAEMOCYTOMETER Table 2 Number of cell in upper grid 15 13 Grid location number Number of cell in lower grid 20 17 1 3 4 10 14 18 13 7 20 15 17 14 13 10 10 12 13 18 Total cells in upper and lower grid 120 120 TOTAL CELLS 240 Total grids 16 Please calculate a numbers of cell/ml from the information table 2.
- There are three main subsystems to a flow cytometer. Describe the primary function of each one in a short phrase or sentence. Please answer with 9 dot points/sentences minimumWhat is the purpose of sheath fluid in a flow cytometer? Please answer with 5 dot points/sentences minimum1) Explain the principle of the electrical impedance cell counting. 2) State 3 Haematology analyzer and their manufacturers. 3) CV for automated instrument is +/- 1% to +/- 2% compared to manual count with CV +/- 10%. Can you explain their differences?
- https://www.youtube.com/watch?v=5EGD39ln5LM Describe the process of loading the hemacytometer, being more specific in how the sample should be loaded.The figures below are the results of flow cytometer analysis of a drugs (camptotechin) effect on cells? From what you learned in class about flowcytometry, briefly state what the drug is doing to the cells: HL60 Untreated Camptothecin (45um, 2h) Camptothecin (45um, 4h) 3.36 10 PI (FL2)You are given a cell stock A. You make a 1:100 dilution and load 10uL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you have 51 cells in average. (Please write down each step)T 1. What is the cell concentration of this diluted sample? 2. What is the cell concentration of original cell stock ? 3. If the original stock A has a total volume of 5mL, what is total number of cells in the A? 4. You decide to do a 1:1000 dilution to verify your counting. After loading and counting, you find out that you have 20 cells in average in each square. Do you think the new concentration is consistent with your previous result? If not, list all the possible errors that can cause this inconsistency. MacBook Air
- Blood plasma flows with a rate of 0.4 cm³/s through a tube with a diameter d = 0.18 cm and length 1 = 1.8 m at a temperature of 20°C. The pressure at the tube entrance is P2 = 1.48 x 104 N/m2. If the temperature of the tube and plasma reaches 29°C, what is the tube entrance pressure P2' needed to maintain the flow rate and pressure at the tube exit constant. Consider 120°c = 1.81 mPa·s and n29°c = 1.28 mPa.s.Choose correct option and explain shortly. Question:- Which of the following is the best description of how you can measure white blood cells (WBCs) using the fluorescent light application of a flow cytometer? (a) How granular each cell is and what surface proteins they express, but not how big they are (b) How granular each cell is, but not how big they are or what surface proteins they express (c) How big each cell is, but not how granular they are or what surface proteins they express (d) How big and how granular each cell is, but not what surface proteins it expresses (e) How big each cell is and what surface proteins they express, but not how granular they are (f) What surface proteins each cell expresses, but not how big or how granular they areA White blood cell Suspension is dyed by Trypan Blue in 1:1 Ratio(Volume). 7 square is counted and the values are; Total Viable Cells: 168 Total Nonviable Cells:32 a.Percentage of Viable Cells: ? b.Average Number Of cells per square: ? c.Dilution Factor:? d.Concentration (Viable Cells/ml): ?