4) List 3 things that a potential vector must have in order to be useful. 5) Sometimes the gene for the enzyme glutathione-S-transferase (GST) is used in purifying your protein of interest. Briefly explain how this is done. 6) What enzyme is used to create CDNA from MRNA?

Q: Does one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?

A: A molecular process in which some discontinuous change to nucleotide sequences in their genomic DNA…

Q: How can we be sure that the insertion of the therapeutic gene does not harm some other necessary…

A: The Food and Drug Administration still has not approved any human gene therapy product. Current gene…

Q: T7 promotor in yeast, your protein is a large complex protein. Which would be the ideal vector to…

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Q: List the three features of a cloning vector and why these elements are needed

A: Cloning vectors act as a vehicle to transfer desired foreign genetic material into a cell. All these…

Q: In gene targeting, homologous recombination between thebacterial chromosome and a linear _______-…

A: Genetic engineering involves the process of using recombinant DNA (rDNA) technology to alter the…

Q: How can I develop a vector to express a bacterial gene (2,500 bp in size) in E. coli. How do I know…

A: Hi! As you have posted multiple questions, I will be answering the first two questions for you. If…

Q: Genetic engineers would like to insert the gene for producing thrombin, a protein that facilitates…

A: Genetic engineering is the process of modifying an organism's genetic makeup using recombinant DNA…

Q: A protein spike of a virus has been identified as an important characteristic of the virus. If the…

A: mRNA technology and polymerase chain reaction can be used to prepare a stable vaccine out of…

Q: Explain how viral vectors can be used for gene therapy, and provide two examples of commonly used…

A: The two methods of gene transfer used in gene therapy are: The viral-mediated gene transfer…

Q: Genetic engineers would like to insert the gene for producing thrombin, a protein which facilitates…

A: Plasmids are circular molecules of DNA. Most plasmids inhabit within bacteria, and around 50% of…

Q: Which of the following viral nucleic acids can be used as a template to produce mRNA? Select all…

A: The information that is stored in the DNA by itself is of no use. There need to be Mechanisms that…

Q: What are transgenic Bacteria? Illustrate using any one example?

A: Genetic engineering is the modification of an organism’s genome for a beneficial outcome. Bacteria,…

Q: Discuss the four important features of DNA vectors.

A: Vector usually is a small DNA molecule which is stable and it can be easily isolated.  A vector…

Q: What are the vectors used in rDNA technology? briefly explain each

A: rDNA technology or Recombinant DNA technology is a widely used technology with the advancement of…

Q: Which of the following is a correct statement about CRISPR-Cas-9 gene editing? Group of answer…

A: CRISPR-Cas9 is a widely used genome editing technology as it enables scientists to alter genes in…

Q: explain how the CRISPR/CAS9  can be used to modify the genome

A: CRISPR-Cas9 is a novel technique that allows geneticists and medical researchers to edit portions of…

Q: How are inactivated vaccine and mRNA vaccine produced? From what you know about DNA technology and…

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Q: Describe the process of cloning a DNA fragment into the EcoR1and AluI sites of the vector pUC18. How…

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Q: In biotechnology procedures, a(n) ____ is a nucleic acid fragment that is used to search for and…

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Q: 2. You are planning to clone a gene into the PBR322 plasmid vector. The gene has Bgl II restriction…

A: Gene cloning is the technique to produce the exact copies of the desired gene by utilizing genetic…

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Q: Based on recent data arising from various animal models, which of the following CRISPR-Cas9 systems…

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Q: Please determine the relative location of the three restriction enzymes. Enzyme Number of fragments…

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Q: In selecting target cells to receive a transferred gene in gene therapy, what factors do you think…

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Q: Does CRISPR CAS9 as a defense in bacteria only work for double stranded viral DNA? or any type of…

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Q: What are the potential concerns regarding the use of viral vectors in gene therapy?

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Q: Consider the reagents required to perform CRISPR genome editing, Which molecule specifies the a new…

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Q: Explain why mRNA is preferred for making COVID-19 vaccines?

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Q: What is the function of the CRISPR/ Cas system 2. Briefly outline the components of the CRISPR/Cas…

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Q: Your PhD thesis advisor has given you the task of preparing a human genomic DNA library.  a. How…

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Q: Contrast the use of plasmid vectors with that of BAC orYAC (bacterial or yeast artificial…

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Genetic Recombination

Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.

Microbial Genetics

Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.