4. In the experiment TLC: (a) When the best separations are achieved, what values range do R, fall between? (3 points) (b) A student analyzed a sample by TLC and developed it with a mixture solvent, 20% methanol in CH2Cl2. The developed TLC plate is shown below. Please improve the separation condition and explain. You don't need to calculate. (5 points) OCH На OCH
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- If the TLC plates had been developed in a more polar solvent, would retention factors on the whole be larger, smaller, or unchanged? Briefly (1 - 2 sentences) explain your reasoning5. Here, you can find an example of a paper chromatography experiment. Use the two Chromatograms below the answer the questions that follow: a) (3p) What are the components of unknown sample (X)? b) (12p) Calculate the Rf values for A, B, C in the first chromatogram. d) (10p) When using the 20% Acetone in Water, are the substances strongly attracted to the stationary phase or the mobile Phase? Briefly explain your reasoning. e) (10p) Which substance does show strongest affinity to the stationary phase in the first chromatogram. Briefly explain your reasoning. wwan www 50 80% Acetone in water 20% Acetone in water Finish line 50 45 45 40 40 35 35 30 30 25 20 15 10 10 Starting line A B C D X A B C D X(6) Ms. Muna Al Naabi in the Analytical chemistry laboratory wanted to separate chlorophenol by liquid chromatography using C-18 column with 10 cm long. She has observed that a chromatogram with compound elute in 9.0 min with W½ = 2.0 min. Find the number of theoretical plate and plate height (in mm).
- 6. This is the TLC plate for the solvent: Ethanol 50% / ethyl acetate 2. 50% V is crystal violet, G is bromocresol green and R is methyl Red. Determine the Rf for each one (Report to 2 # after the decimal) HTML Editor B IUA A 工E三ョ x x 三三 ▼ 目 回 深 T 1 12pt Paragraph 7. 3. 4 5 1. #4The following data give the recovery of bromide from spiked samples of vegetable matter, measuredusing a gas–liquid chromatographic method. The same amount of bromide was added to each specimen(Roughan, J.A., Roughan, P.A. and Wilkins, J.P.G., 1983, Analyst, 108: 742).Tomato: 777 790 759 790 770 758 764 ug/gCucumber: 782 773 778 765 789 797 782 ug/g(a) Test whether the recoveries from the two vegetables have variances which differ significantly.(b) Test whether the mean recovery rates differ significantly.1) Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. STAAT 61 1.11 227 RT TYPE AREA XXXX XXXX XXXX AREAS 0.009 55874 44.117 ARIHT 0.61 XX XX XX 1.11 2.27 XX XX XX TOTAL AREA=XX MUL FACTOR=XX 1. What is the retention time of compound A? Compound B? 2. Which compound is present in a larger amount? 3. Which compound has the lower boiling point? 4. What would happen to the retention times of compounds A and B if the column temperature were raised? 5. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?
- Why is the spectrophotometer set at 100% T using the FeCl3 solution rather than distilled water? O The iron chloride solution is used as the blank bećause aspirin is not soluble in distilled water. The iron chloride solution is used as the blank because distilled water will interfere with the absorbance readings. The iron chloride solution is used as the blank because that is the solvent which is present for all solutions. The iron chloride solution is used as the blank because distilled water has no color.Draw an HPLC instrument diagram by writing the name of the parts. Define the isocratic and gradient system. Write correctly the elution order of the compounds which are shown in the HPLC chromatogram below. Chromatogram Compound B These are called chromatographic peaks and each one represents a separated compound Compound C Point of sample injection into the column Compound AIn a chromatographic separation of two components A and B. Component A has a higher Rf value than component B. This implies that: A. Component B has a higher affinity than A for the mobile phaseB. component B elutes first in the columnC. A has a higher affinity than B for the mobile phaseD. Component A has a higher affinity than B for the stationary phase.
- 1. From spinach, the chromatographic analysis will yield two major substances: (a) B-carotene (C40H56), which will separate from the spinach mixture to form a yellow band, and (b) chlorophyll A (CSSH72MGN4OS), which will separate from the mixture to form a green band. i. Describe the characteristics and draw structures of these molecules. ii. From the spectrophotometric analysis, what wavelength (Amax) would you expect ß-carotene (C40H56) to absorb? What wavelength would chlorophyll A (CssH72MgN4O5) absorb?In TLC, why does the developing solvent 3:1:1 n-butanol/acetic acid/water climb up the TLC plate so slowly?Normal... A T Distribution Table A Chi-Squared Distr... 7. VGR マ This is the TLC plate for the solvent: Ethyl acetate 50%/ 2. hexane 50% V is crystal violet, G is bromocresol green and R is methyl Red. Determine the Re for each one (Report to 2 # after the decimal) ITML Editor 6. 5. 4. 3. 1.