4. Use the cartoon protein below to answer the following questions. Assume the rounder subunits are 45 kDa and the star shaped group is 27 kDa. The lines represent disulfide bonds.

Biology (MindTap Course List)
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Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
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Chapter3: The Chemistry Of Life: Organic Compounds
Section: Chapter Questions
Problem 9TYU: Which of the following levels of protein structure may be affected by hydrogen bonding? (a) primary...
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4. Use the cartoon protein below to answer the following questions. Assume the rounder subunits are 45
kDa and the star shaped group is 27 kDa. The lines represent disulfide bonds.
A. What is the size of the native protein?
B. Draw an SDS-PAGE gel for the sample under reducing and non-
reducing conditions. Clearly label the anode and cathode and label the
lane that contains the reducing sample and the nonreducing sample.
C. Name all of the noncovalent interactions that can occur in holding the star shaped monomer and the spiky
monomers together.
D. What is the most important interaction that holds monomers together in the native structure above?
Transcribed Image Text:4. Use the cartoon protein below to answer the following questions. Assume the rounder subunits are 45 kDa and the star shaped group is 27 kDa. The lines represent disulfide bonds. A. What is the size of the native protein? B. Draw an SDS-PAGE gel for the sample under reducing and non- reducing conditions. Clearly label the anode and cathode and label the lane that contains the reducing sample and the nonreducing sample. C. Name all of the noncovalent interactions that can occur in holding the star shaped monomer and the spiky monomers together. D. What is the most important interaction that holds monomers together in the native structure above?
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