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5) The immunoblot shows probing of samples taken from a time course after treatment with the proteasome inhibitor MG132. Recall that the proteasome is responsible for selectively degrading only those proteins that have been tagged with ubiquitin. The left indicates the antibody that is used to detect protein for each panel. P53 P27 AGR2 GAPDH 0 MG132 (h) 0.5 1 2 4 8 12 24 Image from Wang et al., (2019) Oncogene 38:3458-3474. a) What effect does MG132 have on each protein? What does this tell you about each of these proteins? P53 P27 AGR2 GAPDH

5) The immunoblot shows probing of samples taken from a time course after treatment with the proteasome inhibitor MG132. Recall that the proteasome is responsible for selectively degrading only those proteins that have been tagged with ubiquitin. The left indicates the antibody that is used to detect protein for each panel. P53 P27 AGR2 GAPDH 0 MG132 (h) 0.5 1 2 4 8 12 24 Image from Wang et al., (2019) Oncogene 38:3458-3474. a) What effect does MG132 have on each protein? What does this tell you about each of these proteins? P53 P27 AGR2 GAPDH

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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5) The immunoblot shows probing of samples taken from a time course after treatment with the proteasome inhibitor MG132. Recall that the proteasome is responsible for selectively degrading only those proteins that have been tagged with ubiquitin. The left indicates the antibody that is used to detect protein for each panel. P53 P27 AGR2 GAPDH MG132 (h) 0 0.5 1 2 4 8 12 24 Image from Wang et al., (2019) Oncogene 38:3458-3474. a) What effect does MG132 have on each protein? What does this tell you about each of these proteins? ● P53 P27 AGR2 GAPDH b) How do we know that the differences we observe in the proteins is not due to un- equal loading of samples into each lane of the gel?
Transcribed Image Text:5) The immunoblot shows probing of samples taken from a time course after treatment with the proteasome inhibitor MG132. Recall that the proteasome is responsible for selectively degrading only those proteins that have been tagged with ubiquitin. The left indicates the antibody that is used to detect protein for each panel. P53 P27 AGR2 GAPDH MG132 (h) 0 0.5 1 2 4 8 12 24 Image from Wang et al., (2019) Oncogene 38:3458-3474. a) What effect does MG132 have on each protein? What does this tell you about each of these proteins? ● P53 P27 AGR2 GAPDH b) How do we know that the differences we observe in the proteins is not due to un- equal loading of samples into each lane of the gel?
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