Why is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?
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Why is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?
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- A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which type of bacteria would be used to test the autoclave?A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. What must be done with the bacterial sample after it is processed in the sterilizer?In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?
- Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?Why is the looping out method preferable for sputum specimen for acid fast smears?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?
- What will pose an ethical issue in these procedures are performed - sterilization?Provide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory.During media preparation, you observed that the agar medium did not solidify after sterilization even after incubating it for 1 hour at room temperature. What could be the reason behind this?
- Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation?If cultures are suffering severely due to alkaloid production and accumulation in the medium then, how can i overcome this problem?The acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in performing the acid-fast stain technique because of its thin cell wall. However, if this organism is used for Gram-stain, it can also take the Gram stain reaction. Why is this so?
