5. In the late 1950s, Meselson and Stahl grew bacteria in a medium containing "heavy" nitrogen (15N) and then transferred them to a medium containing 14N. Which of the results in the figure above would be expected after two rounds of DNA replication in the presence of 14N? A) A в) в C) C 14N DNA 14N –15N. hybrid DNA D) D E) E 15N DNA А. В. С. D. E.

5. In the late 1950s, Meselson and Stahl grew bacteria in a medium containing "heavy" nitrogen (15N) and then transferred them to a medium containing 14N. Which of the results in the figure above would be expected after two rounds of DNA replication in the presence of 14N? A) A в) в C) C 14N DNA 14N –15N. hybrid DNA D) D E) E 15N DNA А. В. С. D. E.

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter17: Biotechnology And Genomics

Section: Chapter Questions

Problem 1VCQ: Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner...

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1.If the template DNA sequence is 3' - CCC - ATA - GAG - AAA - 5' , then what is the corresponding daughter strand sequence? A. 5' - GGG - UAU - CUC - UUU - 3 B. 3' - GGG - UAU - CUC - UUU - 5' C. 3' - GGG - TAT - CTC - TTT - 5' D. 5' - GGG - TAT - CTC - TTT - 3'
Describe 5 examples of biological processes that can cause DNA supercoil?
1. Which of the following is the correct order for the steps in DNA extraction? I. Precipitation of DNA with ethanol lI. Treatment with protease to destroy protein and RNAase to destroy RNA III. Disruption of the plasma membrane with detergent IV. Pelleted cell debris forms in a centrifuge a. I, II, III, IV b. III, I, IV, II c. IV, II, I, III d. III, II, IV, I 2. Which of the following group is the most common ancestor of humans and all other vertebrates? a. birds b.fishes c.amphibians d.reptiles
2. Who are the scientists that proved DNA is the transferrable genetic material and Discuss how they proved based on their experiment?
1. How may recombinant DNA molecules be introduced into human cells? a. by splicing the needed genes into a mammalian chromosome using restriction enzymes. b. by adding plasmids to the mammalian cells. c. by using engineered viruses as vectors. d. by using a gene gun. 2. If someone is accused of a crime, investigators can collect his or her DNA to compare the DNA of the cells found at the crime scene. To collect human DNA, investigators often swab the inside of person’s cheek. Just a few human cheek cells contain enough material to perform PCR. In a cell, the nucleus and mitochondria contain DNA that is the starting material for PCR. Identify the 4 components needed to start a PCR reaction (equipment not included)
1. a) If one DNA segment has the following base composition, 5'-CAGTTAGTCA-3', which of the following sequences is complementary? op 1: 3'-CAGTTAGTCA-5' op 2: 3'-TGACTAACTG-5' op 3: 5'-TGACTAACTG-3' op 4: 3'-TGACTAACTG-5' b) What is the nucleotide sequence of the DNA strand that is complementary to 5'-ATCGCAACTGTCACTA-3' op 1: 5'-TAGCGTTGACAGTGATA-3' op 2: 5'-TAGTGACAGTTGCGAT-3' op 3: 5'-ATCACTGTCAACGCTA-3' op 4: 5'-UAGUGACAGUUGCGAU-3'
When Griffith injected mice with a combination of live rough-strain and heat-killed smooth-strain pneumococci, he discovered that (a) the mice were unharmed (b) the dead mice contained living rough-strain bacteria (c) the dead mice contained living smooth-strain bacteria (d) DNA had been transferred from the smooth-strain bacteria to the mice (e) DNA had been transferred from the rough-strain bacteria to the smooth-strain bacteria
2) Considering the technologies that pave the way for the identification of molecules on the basis of hybridization in the classical sense, within the scope of Recombinant DNA Technology; (20P) a) Which of these is used to identify which macromolecule,b) What do you understand in the context of hybridization and between which molecules there are technique-specificinteractions take placec) “Nylon membrane” or “nitrosdulose” commonly used in these technologieswhy "membranes" are needed,d) Explain how to design an application example for each technique you mentioned.
8 How natural processes can change the information stored in DNA?
Mention two functions of DNA polymerase I in E. coli replication machinery?
4. What is the name of the process by which bacteria pick up a different organism’s geneticmaterial?5. Genetically, how does the original bacterial DNA (plasmid) differ from the final bacterial DNAmolecule? (Do not say, “It is longer.”)6. Tetracycline is an antibiotic that is prescribed to kill bacterial infections. The transformedbacterial cell that you created has the tetracycline resistant gene in it. If this cell is placed on agrowth medium with tetracycline, will the bacteria grow or die? Explain.
4. Bacterial transformation and bacteriophage labelingexperiments proved that DNA was the hereditary material in bacteria and in DNA-containing viruses.Some viruses do not contain DNA but have RNA inside the phage particle. An example is the tobaccomosaic virus (TMV) that infects tobacco plants, causing lesions in the leaves.Two different variants of TMV exist that havedifferent forms of a particular protein in the virus particle that can be distinguished. It is possible to reconstitute TMV in vitro (in the test tube) by mixing purifiedproteins and RNA. The reconstituted virus can then beused to infect the host plant cells and produce a newgeneration of viruses. Design an experiment to showthat RNA, rather than protein, acts as the hereditarymaterial in TMV.