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Q7. Meselson and Stahl conducted an experiment to prove that the replication of DNA is semi-conservative. 3.2, 3.3,) (A.C. (a) Replication of DNA is described as 'semi-conservative'. Explain this term. (b) Explain how the DNA molecule replicates. (c) Meselson and Stahl's experiments involved growing bacteria in culture media containing either heavy nitrogen (15N) or light nitrogen (14N). The DNA was then extracted from the bacteria. The table below summarises the three stages of Meselson and Stahl's experiment and their results. (a) Complete the table by drawing, in the appropriate boxes, diagrams of the DNA molecules and mark the position and size of the DNA bands in the tubes. Experimental stage Diagram to show the strands in the DNA molecules of the bacteria separating solution Position and size of DNA bands in the tube of Stage 1 Bacteria grown for several generations in culture medium containing heavy nitrogen Stage 2 The bacteria from the end of stage 1 were grown for Janother generation in culture medium containing light nitrogen Stage 3 The bacteria from the end of stage 2 were grown for one more generation in culture medium containing light nitrogen (b) The bacteria at the end of stage two were grown for five more generations. After each generation, what would you expect to see in the test tube? Draw these in the test tubes below. Gen. 0 Gen. 1 Gen. 2 Gen.3 Gen.4

Q7. Meselson and Stahl conducted an experiment to prove that the replication of DNA is semi-conservative. 3.2, 3.3,) (A.C. (a) Replication of DNA is described as 'semi-conservative'. Explain this term. (b) Explain how the DNA molecule replicates. (c) Meselson and Stahl's experiments involved growing bacteria in culture media containing either heavy nitrogen (15N) or light nitrogen (14N). The DNA was then extracted from the bacteria. The table below summarises the three stages of Meselson and Stahl's experiment and their results. (a) Complete the table by drawing, in the appropriate boxes, diagrams of the DNA molecules and mark the position and size of the DNA bands in the tubes. Experimental stage Diagram to show the strands in the DNA molecules of the bacteria separating solution Position and size of DNA bands in the tube of Stage 1 Bacteria grown for several generations in culture medium containing heavy nitrogen Stage 2 The bacteria from the end of stage 1 were grown for Janother generation in culture medium containing light nitrogen Stage 3 The bacteria from the end of stage 2 were grown for one more generation in culture medium containing light nitrogen (b) The bacteria at the end of stage two were grown for five more generations. After each generation, what would you expect to see in the test tube? Draw these in the test tubes below. Gen. 0 Gen. 1 Gen. 2 Gen.3 Gen.4

Biology 2e
Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter14: Dna Structure And Function
Section: Chapter Questions
Problem 17RQ: Which of the following is not a true statement comparing prokaryotic and eukaryotic DNA replication?...
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Q7. Meselson and Stahl conducted an experiment to prove that the replication of DNA is semi-conservative. 3.2, 3.3,) (A.C. (a) Replication of DNA is described as 'semi-conservative'. Explain this term. (b) Explain how the DNA molecule replicates. (c) Meselson and Stahl's experiments involved growing bacteria in culture media containing either heavy nitrogen (15N) or light nitrogen (14N). The DNA was then extracted from the bacteria. The table below summarises the three stages of Meselson and Stahl's experiment and their results. (a) Complete the table by drawing, in the appropriate boxes, diagrams of the DNA molecules and mark the position and size of the DNA bands in the tubes. Experimental stage Diagram to show the strands in the DNA molecules of the bacteria separating solution Position and size of DNA bands in the tube of Stage 1 Bacteria grown for several generations in culture medium containing heavy nitrogen Stage 2 The bacteria from the end of stage 1 were grown for another generation in culture medium containing light nitrogen Stage 3 The bacteria from the end of stage 2 were grown for one more generation in culture medium containing light nitrogen (b) The bacteria at the end of stage two were grown for five more generations. After each generation, what would you expect to see in the test tube? Draw these in the test tubes below. Gen. 0 Gen. 1 Gen. 2 Gen.3 Gen.4 (c) Which part of the DNA molecule would be expected to incorporate the nitrogen?
Transcribed Image Text:Q7. Meselson and Stahl conducted an experiment to prove that the replication of DNA is semi-conservative. 3.2, 3.3,) (A.C. (a) Replication of DNA is described as 'semi-conservative'. Explain this term. (b) Explain how the DNA molecule replicates. (c) Meselson and Stahl's experiments involved growing bacteria in culture media containing either heavy nitrogen (15N) or light nitrogen (14N). The DNA was then extracted from the bacteria. The table below summarises the three stages of Meselson and Stahl's experiment and their results. (a) Complete the table by drawing, in the appropriate boxes, diagrams of the DNA molecules and mark the position and size of the DNA bands in the tubes. Experimental stage Diagram to show the strands in the DNA molecules of the bacteria separating solution Position and size of DNA bands in the tube of Stage 1 Bacteria grown for several generations in culture medium containing heavy nitrogen Stage 2 The bacteria from the end of stage 1 were grown for another generation in culture medium containing light nitrogen Stage 3 The bacteria from the end of stage 2 were grown for one more generation in culture medium containing light nitrogen (b) The bacteria at the end of stage two were grown for five more generations. After each generation, what would you expect to see in the test tube? Draw these in the test tubes below. Gen. 0 Gen. 1 Gen. 2 Gen.3 Gen.4 (c) Which part of the DNA molecule would be expected to incorporate the nitrogen?
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