5. Provided with the following data, compute the corresponding CFU/ml of the original culture. Assume that spread plate technique was done. Show your solutions.
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- 2.1The number of bacteria in the samples need to be quantified. The laboratory uses a specificprotocol where the dilution factor on the spread plates must be:10-6; 10-7; 10-8Describe how you would prepare the required dilution series using the least amount of dilutionwater. 2.2 Calculate the amount of bacteria in the sample from the following results (show the steps in the calculation): Dilution factor Count10-6 27110-7 3510-8 21A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-2 plate? Include units and use OCD formula.0.2 ml of culture . Please explain solution
- Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.204 and A650=0.061, taking into account the dilution factor as per question 6, and the total volume of extract as per question 4. Note your answer to 2 decimal places Sample 1 Total Extract Volume (ml) is 45 Dilution factor is 100 A280 is 1.07 A620 is 0.221 A650 is 00.97 Specific Absorbance Ratio (SAR) A620/A280 is 0.2061. One gram of sludge was added to a 99 mL dilution blank. Three 1/10 dilutions were then made. From the last dilution made, 200 µL was plated onto an all-purpose medium. After incubation, 123 colonies were counted. What is the CFU/mL in the sludge? Note: 1 gram sludge is equal to 1 mL sludge.Design a serial dilution of a sample to achieve a A) Final dilution of 10-4 using 9.0 mL blanks B) Final dilution of 10-6 using 99 mL blanks C) Final dilution of 10-6 using 9.0 mL blanks
- In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperAndrew has to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria Bertani Agar. a. What is the total volume of the medium needed? b. What is the amount of each component needed, given the following media composition per liter of distilled water:1. Calculate the CFU/mL of the original culture for the countable plate as shown in the diagram. b) How many colonies would you expect if you plated out 0.1 mL from Tube C. c) How many colonies would you expect if you plated out 1.0 mL from Tube C.
- Illustrate the different solid tubed media formed 1. Deep/Butt solid tubed media2. Slant solid tubed media 3. Butt-slant solid tubed mediaAnswer the ff with not more than two sentences: Why should Erlenmeyer flasks hold not more than 60% of their capacity? When can gelatin be used in culture media preparation? How does cotton plug prevent contamination of the medium? Why should caps of glassware be loosened first before sterilization?Please explain Now that the laboratory scientist has a 1:10 dilution, what dilution should he make next to create a 1:100 dilution? Choose best letter answer and give a brief explanation. A. 1:2 B. 1:5 C. 1:10 D. 1:100