Need help with question 5 and 6 Exercise II4. Complete the following table to calculate what volume of primers to add to your PCR master mix:Per SampleMaster MixAdded toReagent1X3.5XMaster Mix?Water19.9 ul69.7 µlBuffer (10X with Mg2+)2.5 μι8.8 μForward Primer (M13F), 10 µM1 ul3.5Reverse Primer (M13R), 10 µM1 ul3.5DNTPS (10 mM each, 40 mM total)0.5 ul1.8 μιTaq Polymerase0.1 pl0.4 ulTotal Volume25 μι87.5 µl50Have your Lab Instructor check your calculations before you add any reagents to your master mix.W Lab Instructor Initials:5. Suppose that you made a mistake when you set up the thermocycler program and omitted thefirst 2 minutes at 95°C. How would this impact your PCR?6. Suppose that you made a mistake when you set up the thermocycler program and onlyprogrammed for 10 cycles instead of 30 cycles. How would this impact your PCR?

Answer: 5 : the first 2 minutes at 95 degree Celsius is needed to open the dna if this is omitted…

5. Suppose that you made a mistake when you set up the thermocycler program and omitted the first 2 minutes at 95°C. How would this impact your PCR? 5. Suppose that you made a mistake when you set up the thermocycler program and only programmed for 10 cycles instead of 30 cycles. How would this impact your PCR?

5. Suppose that you made a mistake when you set up the thermocycler program and omitted the first 2 minutes at 95°C. How would this impact your PCR? 5. Suppose that you made a mistake when you set up the thermocycler program and only programmed for 10 cycles instead of 30 cycles. How would this impact your PCR?

Comprehensive Medical Terminology

4th Edition

ISBN:9781133478850

Author:Jones

Publisher:Jones

Chapter21: Oncology (cancer Medicine)

Section: Chapter Questions

Problem F1CRE

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