5.3 buffer -pH 3.3 buffer pH 4.3 buffer Asp Thr Glu Ser Ala His Met lle Тyr Gly Phe Lys Leu NH, Val, Arg Pro 40 80 120 160 200 240 280 320 330 370 410 450 490 550 590 630 Effluent (mL) Absorbance |||| | |

Appl Of Ms Excel In Analytical Chemistry
2nd Edition
ISBN:9781285686691
Author:Crouch
Publisher:Crouch
Chapter8: Polyfunctional Acids And Bases
Section: Chapter Questions
Problem 6P
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Why are buffer solutions of increasingly higher pH used to elute the column that generates the chromatogram shown in Figure

5.3 buffer
-pH 3.3 buffer
pH 4.3 buffer
Asp
Thr
Glu
Ser
Ala
His
Met lle
Тyr
Gly
Phe
Lys
Leu
NH,
Val,
Arg
Pro
40
80
120 160 200 240
280 320 330 370
410
450
490 550 590 630
Effluent (mL)
Absorbance
|||| | |
Transcribed Image Text:5.3 buffer -pH 3.3 buffer pH 4.3 buffer Asp Thr Glu Ser Ala His Met lle Тyr Gly Phe Lys Leu NH, Val, Arg Pro 40 80 120 160 200 240 280 320 330 370 410 450 490 550 590 630 Effluent (mL) Absorbance |||| | |
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