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- 28. In addition to what is not a characteristic of gas chromatography as listed in the probelm can you give the general characteristics of Gas Chromatography?In column chromatography, dyes with higher polarities tend to attach to which of the forbwing? A. THF-Acetone B. Silica C. Acetone D. TetrahydrofuranSuggest two possible applications for paper chromatography other than the separation of dyes or the identification of metal ions.
- During chromatographic analysis of bromocamphor, two peaks were found on the chromatogram: camphor (allowable impurity) with an area of 57 mm2 and bromocamphor- 1929 mm2. Calculate the content of bromocamphor in the sample.Chromatography is a separation technique. What is the physical or chemical basis for separation of compounds during thin layer chromatography?3. A student separated an unknown mixture of ions using the procedure of Part A. When the spots were made visible, a faint spot corresponding to Cu2+was seen. However, the student later learned that the unknown did not contain Cu2+, Which of the following could cause this result?a. The same spotting capillary was used to spot the Cu2+sample and the unknown sample.b. The Cu2+sample was contaminated with Co2+and Fe3+c. The chromatography paper was not trimmed to the correct dimensions.d. The position of the mobil-phase front was incorrectly marked.Explain your answer.
- Doe hydrophobic silica gel act as non-polar stationary phase in TLC making the process as reverse chromatography? explainThe following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67Tell me about the Laboratory experiment: Purifying Protein by Column Chromatography Thank you^^
- [7] Which of the following are necessary steps in performing paper chromatography? I. Saturate the chamber with the vapor of the mobile phase II. Allow the chromatogram to touch the sides of the beaker III. Place the spots at the same height as the solvents IV. Spot the samples 2 cm apart from each other27. What exactly is meant by reversed phase? In addition to finding what compound would elute first in a reverse phase HPLC Separation... Can you explain what would elute first for HPLC, GC, TLC, IEC, SEC (or gel filtration) and affinity chromatography?How might the Rf values of colorless substances be determined? That is, how could the colorless spots be located? (for paper chromatography)