7]  Which of the following are necessary steps in performing paper chromatography?    I. Saturate the chamber with the vapor of the mobile phase    II. Allow the chromatogram to touch the sides of the beaker    III. Place the spots at the same height as the solvents    IV. Spot the samples 2 cm apart from each other

EBK A SMALL SCALE APPROACH TO ORGANIC L
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Chapter20: Nucleophilic Substitution Reactions: Competing Nucleophiles
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[7] 

Which of the following are necessary steps in performing paper chromatography?
   I. Saturate the chamber with the vapor of the mobile phase
   II. Allow the chromatogram to touch the sides of the beaker
   III. Place the spots at the same height as the solvents
   IV. Spot the samples 2 cm apart from each other
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