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6. Follow the directions from Investigation 5 for the following sequence: HUMAN GENOMIC SEQUENCE (MAP 1 > 600) SITE AND SEQUENCE ENZYMES 5'-GATTCCGTGGCGAGACGATOGTTGAAAAGTCGCGCGCGCTACTAAAGCTGOCAMAATGGA-31 3'-CTAAGGCACCGCTCTGCTAGCAACT 5'-AGTAAACACAGTTATTTGATTTICA 3'-TCATTTGTGTCAATAAACTAAAM 5'-ATTTTTGACTGGTCTACT WAA *********** +|+ TTTCGACCGTTTTACCT-5' TTAGGATOGATCGGTTT-3' + ++++++++ 3'-TAAAAACTGACCAGATGATTGCAAAAAAAAACGGAAAGCAAAAGTGTCAACCAATAGAGT-5' 5'-GAGTTGGGAAGTTACTCGGTA CCAAA-5 TOGTTATCTCA-3 +----+----+-- 5'-ATTAGTGCCTTAATAGTAGAGATAAATTTGGCAAGGTAAGTAACCAAAACAAATTGTTTA-3' 3'-TAATCACGGAATTATCATCTCTATTTAAACCGTTCCATTCATTGGTTTTGTTTAACAAAT-5¹ 5'-TTTAACAAACAGACGAACAGTGACCTCGTTTACTAACTAATGATTCTTCTATTACTTGTT-3 3'-AAATTGTTTGTCTGCTTGTCACTGGAGCAAATGATTGATTACTAAGAAGATAATGAACAA-5' 5'-GCAGCGTCGAAGGGGCGAGTTAATTTACGCCATCAAGGAGGCATGGTCAACCCCCAAAAC-3' ******fn||n||n|| 3'-CGTCGCAGCTTCCCCGCTCAATTAAATGCGGTAGTTCCTCCGTACCAGTTGGGGGTTTTG-5 5'-CCCTGGAAATTTCGAATAAATGTTAACAATTTTATATAAGAAACCAAAACATTTAATTTT-3¹ ************|-|-|-*************** 3'-GGGACCTTTAAAGCTTATTTACAATTGTTAAAATATATTCTTTGGTTTTGTAAATTAAAA-5' LATCAACA-3 3'-CTCAACCCTTCAATGAGCCATCGACGTTTTTGTAGCTGCTATAGTTTGGTGTTTAGTTGT-5' 60 120 180 240 300 360 420 480 540 600 7. Please write the message you have decoded here (using single letter amino acid symbols): Lab Report DNA and BLAST 139

6. Follow the directions from Investigation 5 for the following sequence: HUMAN GENOMIC SEQUENCE (MAP 1 > 600) SITE AND SEQUENCE ENZYMES 5'-GATTCCGTGGCGAGACGATOGTTGAAAAGTCGCGCGCGCTACTAAAGCTGOCAMAATGGA-31 3'-CTAAGGCACCGCTCTGCTAGCAACT 5'-AGTAAACACAGTTATTTGATTTICA 3'-TCATTTGTGTCAATAAACTAAAM 5'-ATTTTTGACTGGTCTACT WAA *********** +|+ TTTCGACCGTTTTACCT-5' TTAGGATOGATCGGTTT-3' + ++++++++ 3'-TAAAAACTGACCAGATGATTGCAAAAAAAAACGGAAAGCAAAAGTGTCAACCAATAGAGT-5' 5'-GAGTTGGGAAGTTACTCGGTA CCAAA-5 TOGTTATCTCA-3 +----+----+-- 5'-ATTAGTGCCTTAATAGTAGAGATAAATTTGGCAAGGTAAGTAACCAAAACAAATTGTTTA-3' 3'-TAATCACGGAATTATCATCTCTATTTAAACCGTTCCATTCATTGGTTTTGTTTAACAAAT-5¹ 5'-TTTAACAAACAGACGAACAGTGACCTCGTTTACTAACTAATGATTCTTCTATTACTTGTT-3 3'-AAATTGTTTGTCTGCTTGTCACTGGAGCAAATGATTGATTACTAAGAAGATAATGAACAA-5' 5'-GCAGCGTCGAAGGGGCGAGTTAATTTACGCCATCAAGGAGGCATGGTCAACCCCCAAAAC-3' ******fn||n||n|| 3'-CGTCGCAGCTTCCCCGCTCAATTAAATGCGGTAGTTCCTCCGTACCAGTTGGGGGTTTTG-5 5'-CCCTGGAAATTTCGAATAAATGTTAACAATTTTATATAAGAAACCAAAACATTTAATTTT-3¹ ************|-|-|-*************** 3'-GGGACCTTTAAAGCTTATTTACAATTGTTAAAATATATTCTTTGGTTTTGTAAATTAAAA-5' LATCAACA-3 3'-CTCAACCCTTCAATGAGCCATCGACGTTTTTGTAGCTGCTATAGTTTGGTGTTTAGTTGT-5' 60 120 180 240 300 360 420 480 540 600 7. Please write the message you have decoded here (using single letter amino acid symbols): Lab Report DNA and BLAST 139

Biology: The Dynamic Science (MindTap Course List)
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter14: Dna Structure And Replication
Section: Chapter Questions
Problem 7TYK
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6. Follow the directions from Investigation 5 for the following sequence: HUMAN GENOMIC SEQUENCE (MAP 1 > 600) SITE AND SEQUENCE ENZYMES 60 5'-GACTATTCTACGGCGCTCCCAACGATCAAAATGTGTGCCAAAAAAAAGCAGCGAAACGCA-3' ▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬ 3'-CTGATAAGATGO GTTGCTAGTTTTACACACGGTTTTTTTTCGTCGCTTTGCGT-5¹ 5-G ****|-*-++++++|+++++++++|++++|---|--- CGCG 5'-ACGGACGTAATTTTCATTATAATTACTCCTTATCGATCATTGGCAGTTCAGGTTGTTTGG-3¹ **********|-|-▬▬▬▬-|-***÷----|-+*+---+-- 3¹-TGCCTGCATTAAAAGTAATATTAATGAGGAATAGCTAGTAACCGTCAAGTCCAACAAACC-5¹ 5'-GATTCCGTGGCGAGACGATCGTTGAAAAGT SAAAAGTCGCGCGCGCTACTAAAGCTGGCAAAATGGA-3' 3'-CTAAGGCACCGCTCTGCTAGCAACTTTTC 5'-AGTAAACACAGTTATTIGATTY GCGATGATTTCGACCGTTTTACCT-5' TTTGATTTTCACTTTCGTTTAAATTATTTTTAGGATCGATCGGTTT-3' ************|*|*|*▬▬▬f=▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬ 3'-TCATTTGTGTCAATAAACTAAAAGTGAAAGCAAATTTAATAAAAATCCTAGCTAGCCAAA-5' 5'-ATTTTTGACTGGTCTACTAACGTTTTTTTTTGCCTTTCGTTTTCACAGTTGGTTATCTCA-3' 3'-TAAAAACTGACCAGATGATTGCAAAAAAAAACGGAAAGCAAAAGTGTCAACCAATAGAGT-5¹ 5'-ATTAGTGCCTTAATAGTAGAGATAAATTTGGCAAGGTAAGTAACCAAAACAAATTGTTTA-3' ***********…|-|-**************** 3'-TAATCACGGAATTATCATCTCTATTTAAACCGTTCCATTCATTGGTTTTGTTTAACAAAT-5' 5'-TTTAACAAACAGACGAACAGTGACCTCGTTTACTAACTAATGATTCTTCTATTACTTGTT-3' *************þuntfp|nn|nz|n|| 3'-AAATTGTTTGTCTGCTTGTCACTGGAGCAAATGATTGATTACTAAGAAGATAATGAACAA-5' 5'-GCAGCGTCGAAGGGGCGAGTTA TTTACGCCATCAAGGAGGCATGGTCAACCCCCAAAAC-3' 3'-CGTCGCAGCTTCCCCGCT ATTAAATGCGGTAGTTCCTCCGTACCAGTTGGGGGTTTTG-5¹ ************|*|*|*|*|*|*|*|*|-| 5'-CCCTGGAAATTTCGAATAAATGTTAACAATTTTATATAAGAAACCAAAACATTTAATTTT-3' 3'-GGGACCTTTAAAGCTTATTTACAATTGTTAAAATATATTCTTTGGTTTTGTAAATTAAAA-5¹ 5'-GAGTTGGGAAGTTACTCGGTAGCTGCAAAAACATCGACGATATCAAACCACAAATCAACA-3' 3'-CTCAACCCTTCAATGAGCCATCGACGTTTTTGTAGCTGCTATAGTTTGGTGTTTAGTTGT-5' 120 300 180 240 300 360 420 480 540 600 7. Please write the message you have decoded here (using single letter amino acid symbols): Lab Report DNA and BLAST 139
Transcribed Image Text:6. Follow the directions from Investigation 5 for the following sequence: HUMAN GENOMIC SEQUENCE (MAP 1 > 600) SITE AND SEQUENCE ENZYMES 60 5'-GACTATTCTACGGCGCTCCCAACGATCAAAATGTGTGCCAAAAAAAAGCAGCGAAACGCA-3' ▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬ 3'-CTGATAAGATGO GTTGCTAGTTTTACACACGGTTTTTTTTCGTCGCTTTGCGT-5¹ 5-G ****|-*-++++++|+++++++++|++++|---|--- CGCG 5'-ACGGACGTAATTTTCATTATAATTACTCCTTATCGATCATTGGCAGTTCAGGTTGTTTGG-3¹ **********|-|-▬▬▬▬-|-***÷----|-+*+---+-- 3¹-TGCCTGCATTAAAAGTAATATTAATGAGGAATAGCTAGTAACCGTCAAGTCCAACAAACC-5¹ 5'-GATTCCGTGGCGAGACGATCGTTGAAAAGT SAAAAGTCGCGCGCGCTACTAAAGCTGGCAAAATGGA-3' 3'-CTAAGGCACCGCTCTGCTAGCAACTTTTC 5'-AGTAAACACAGTTATTIGATTY GCGATGATTTCGACCGTTTTACCT-5' TTTGATTTTCACTTTCGTTTAAATTATTTTTAGGATCGATCGGTTT-3' ************|*|*|*▬▬▬f=▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬ 3'-TCATTTGTGTCAATAAACTAAAAGTGAAAGCAAATTTAATAAAAATCCTAGCTAGCCAAA-5' 5'-ATTTTTGACTGGTCTACTAACGTTTTTTTTTGCCTTTCGTTTTCACAGTTGGTTATCTCA-3' 3'-TAAAAACTGACCAGATGATTGCAAAAAAAAACGGAAAGCAAAAGTGTCAACCAATAGAGT-5¹ 5'-ATTAGTGCCTTAATAGTAGAGATAAATTTGGCAAGGTAAGTAACCAAAACAAATTGTTTA-3' ***********…|-|-**************** 3'-TAATCACGGAATTATCATCTCTATTTAAACCGTTCCATTCATTGGTTTTGTTTAACAAAT-5' 5'-TTTAACAAACAGACGAACAGTGACCTCGTTTACTAACTAATGATTCTTCTATTACTTGTT-3' *************þuntfp|nn|nz|n|| 3'-AAATTGTTTGTCTGCTTGTCACTGGAGCAAATGATTGATTACTAAGAAGATAATGAACAA-5' 5'-GCAGCGTCGAAGGGGCGAGTTA TTTACGCCATCAAGGAGGCATGGTCAACCCCCAAAAC-3' 3'-CGTCGCAGCTTCCCCGCT ATTAAATGCGGTAGTTCCTCCGTACCAGTTGGGGGTTTTG-5¹ ************|*|*|*|*|*|*|*|*|-| 5'-CCCTGGAAATTTCGAATAAATGTTAACAATTTTATATAAGAAACCAAAACATTTAATTTT-3' 3'-GGGACCTTTAAAGCTTATTTACAATTGTTAAAATATATTCTTTGGTTTTGTAAATTAAAA-5¹ 5'-GAGTTGGGAAGTTACTCGGTAGCTGCAAAAACATCGACGATATCAAACCACAAATCAACA-3' 3'-CTCAACCCTTCAATGAGCCATCGACGTTTTTGTAGCTGCTATAGTTTGGTGTTTAGTTGT-5' 120 300 180 240 300 360 420 480 540 600 7. Please write the message you have decoded here (using single letter amino acid symbols): Lab Report DNA and BLAST 139
ment Lopez Chun 1 1006 is Diego 5. For the following DNA strand, complete the following: a. Write the complementary DNA strand below the strand. 5'-A CCTA CGTTC GACGTA ACCGCAT T-3' 3-T6G AT GCAAG CTGCATT GG C6TAA_5² b. Replicate the DNA strand (remember semiconservative replication). 5'-AC CTA CGTTCGAC GTAA CCGCATT-3' 3'- 5'- 3'- 5'-ACCGTTCGACGTAACCGCATT-3' - 5' 138 DNA and BLAST Lab Report - 3' - 5' OLD NEW NEW OLD c. Transcribe the 5' to 3'DNA to an mRNA strand (remember there is NO thymine in RNA). 5'-A CCTA CGTTCGACGTA ACCG CATT-3' DNA mRNA d. Take mRNA strand and convert to amino acid (protein) using mRNA codon table (Table 4). Remember mRNA is read 5' to 3' so you will need to re-write the sequence to begin with the 5'end. Start protein at the AUG sequence. mRNA Protein
Transcribed Image Text:ment Lopez Chun 1 1006 is Diego 5. For the following DNA strand, complete the following: a. Write the complementary DNA strand below the strand. 5'-A CCTA CGTTC GACGTA ACCGCAT T-3' 3-T6G AT GCAAG CTGCATT GG C6TAA_5² b. Replicate the DNA strand (remember semiconservative replication). 5'-AC CTA CGTTCGAC GTAA CCGCATT-3' 3'- 5'- 3'- 5'-ACCGTTCGACGTAACCGCATT-3' - 5' 138 DNA and BLAST Lab Report - 3' - 5' OLD NEW NEW OLD c. Transcribe the 5' to 3'DNA to an mRNA strand (remember there is NO thymine in RNA). 5'-A CCTA CGTTCGACGTA ACCG CATT-3' DNA mRNA d. Take mRNA strand and convert to amino acid (protein) using mRNA codon table (Table 4). Remember mRNA is read 5' to 3' so you will need to re-write the sequence to begin with the 5'end. Start protein at the AUG sequence. mRNA Protein
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