6a. C. elegans display a very characteristic sinusoidal movement, like a snake slithering. Imagine you complete a genetic screen in C. elegans for immobile worms, that lack this ability to move. These sorts of mutants belong to a class of mutants called "unc" mutants, which stands for "uncoordinated." Unc mutants exhibit some degree of paralysis from minor to severe, and they are typically not lethal. Imagine you create a recessive mutation in the unc-1 gene, through a mutagenesis. You initially collect a heterozygote: unc-1(-)/+ ( unc-1(-) is the loss-of-function mutation and “+” represents the wild type allele at that gene). You allow the C.elegans mutant to self-fertilize. What percentage of the offspring would now be homozygous for the mutation? 6b. Say you've only been working with your unc-1(-) mutant at 26 degrees Celsius, the restrictive condition. What experiment would you do and what result would expect if the mutant you were dealing with was temperature-sensitive?

6a. C. elegans display a very characteristic sinusoidal movement, like a snake slithering. Imagine you complete a genetic screen in C. elegans for immobile worms, that lack this ability to move. These sorts of mutants belong to a class of mutants called "unc" mutants, which stands for "uncoordinated." Unc mutants exhibit some degree of paralysis from minor to severe, and they are typically not lethal. Imagine you create a recessive mutation in the unc-1 gene, through a mutagenesis. You initially collect a heterozygote: unc-1(-)/+ ( unc-1(-) is the loss-of-function mutation and “+” represents the wild type allele at that gene). You allow the C.elegans mutant to self-fertilize. What percentage of the offspring would now be homozygous for the mutation? 6b. Say you've only been working with your unc-1(-) mutant at 26 degrees Celsius, the restrictive condition. What experiment would you do and what result would expect if the mutant you were dealing with was temperature-sensitive?

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 2DID

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