7. Assuming that these are the 12 microplates. What is wrong with the result of the test? What do you think the cause of this occurrence? What should the medical technologist do? ‒‒‒‒‒‒• 100000000 AA AA AA for number 7
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- ELISAs require a number of steps to be completed in order for the test to work. Why do you need to wash your samples at each step? When you added primary antibody to the wells, what happened if your sample contained the antigen? What if it did not contain the antigen? When you added secondary antibody to the wells, what happened if your sample contained the antigen? What if it did not contain the antigen?Chemistry I have a question relate to ELISA If the student inject the booster antigen 1 month after the initial inoculation, and collect antibodies (antiserum) 1 week later, what kind of antibodies does the student expect to find?serology (elisa) lab: When you added primary antibody to the wells, what happened if your sample contained the antigen? What if it did not contain the antigen?
- serology (elisa) lab: When you added secondary antibody to the wells, what happened if your sample contained the antigen? What if it did not contain the antigen?serology (elisa) lab: What antibody-based tests can you buy at your local pharmacy?1. You are asked to make 150 ml of 2% agarose using a well-known buffer. How many grams will be used? a) 0.03 g b) 0.3 g c) 3 g d) 30 g 2. Place the following reactants in their proper order for the indirect ELISA test 1 = enzyme-linked antibody 2 = known antigen 3 = patient serum 4 = substrate
- What other test can be used to confirm the results obtained for a TEV infected tomato in an ELISA test? Name and very briefly describe one other test. Give the reason for your choice.In a direct ELISA, which of the following does the patient provide? A.) Substrate B.) Enzymes C.) Antibodies D.) AntigensYou perform an ELISA to test hospital soap samples for contamination with Pseudomonas aeruginosa. Other than the different molecule of interest, this ELISA works exactly like the one you saw in the video. You run the soap sample, a negative control, and a positive control, all in duplicate. How would you interpret the following results? Laboratory Notebook: ELISA – Pseudomonas aeruginosa Well Contents Observation at 15 min Result 1 Negative control 2 Soap sample 3 Positive control 4 Negative control 5 Soap sample 6 Positive control 7 Empty well 8 Empty well The soap is POSITIVE for the Pseudomonas aeruginosa antigen. You cannot interpret the soap data, because the NEGATIVE control didn’t work. The soap is NEGATIVE for the Pseudomonas aeruginosa antigen. You cannot interpret the soap data, because the POSITIVE control didn’t work.
- serology (elisa) lab: What problems can prevent the immune system from working properly?All of the following are used in a direct ELISA, except: A) Primary enzyme linked antibody B) Substrate C) Secondary enzyme linked antibody D) Primary antibodyserology (elisa) lab: The samples that you added to the microplate strip contain many proteins and may or may not contain the disease antigen. What happened to the proteins in the plastic well if the sample contained the antigen? What if it did not contain the antigen?