8. What is the optimum pH to separate a mixture of lysine (2.2, 9,0, 10.0), arginine (1.82, 8.99, 13.2), and cysteine (1.8, 10.8, 8.3) by spotting the mixture in the center of a piece of paper and subjecting it to an electric field? A. 4.5 В. 5.0 с. 7.0 D. 7.5 E. 8.0 F. 8.5 G. 5.5 Н. 9.5 I. 11.0
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- Alanine is a diprotic amino acid with a pKa = 2.344 for the carboxylic group and a pKa = 9.868 for the ammonium group. Estimate the pH of a solution of 0.340 M alanine. Answer using two significant figures.If the concentration of an acid in solution is 2.2 times larger than the concentration of its conjugate base, and the pH of the solution is 3.5, what is the pKa of the acid? Include the answer to 3 significant figuresPhenolphthalein has a pKa of 9.7 and is colorless in its acid form and pink in its basic form. For pH= 7.7 calculate [In−]/[HIn]. Express your answer using two significant figures.
- The Ka of the conjugate acid of a pesticide is 6.7 x 10-6. What is the Kb of the pesticide at 25°C? 1.5 x 1019 1.5 x 10-9 6.7 x 10-8 6.7 x 10-20The measured pH of a 0.100 M solution of triethylamine (NEt3) is 11.68. What pKa of triethylammonium (HNEt3+) is implied by this data? Enter your response to the nearest 0.01.A solution of amino acid having carboxylic side chain was titrated against NaOH. If initial pH of the solution was 2.8. Describe the state of the functional groups of the amino acid at the initial pH and at 10.5.
- 1) You are asked to prepare a buffer for a protein assay. This protein works best at a slightly acidic pH of 5.5. a. From the list of Acids and conjugate bases which pair would you pick. (The list is in the attached image) b. You are asked to make a 1M stock solu on of the Buffer, how many grams of each acid and base would you need to make a 500mL stock. c. For the experiment, the final concentra on of the buffer should be 50mM. How many mL of your stock would you need to make 2L. Hint: M1V1=M2V2In the preparation of the standard curve for protein analysis, 50 mn BSA (bovine serum albumin) dissolved in H2O to a final volume of 5 mL was used as stock solution. What is the weight of BSA in 0.1 mL of stock solution? In 0.2 mL?4. You have a solution of leucine at 0.1 gram in 100 ml. Give (a) the molar concentrations and (b) the structures of each ionic form of leucine that will be present at pH 7.4. The pKa values for leucine are 2.36 and 9.6. Please type answer note write by hend.
- For a weak acid with a pKa of 6.0, show how you would calculate the ration of acid to salt at pH 5.What is the pK of the weak acid HA if a solution containing 0.1 M HA and 0.2 M A− has a pH of 6.5?The pKa of a weak acid was determined by measuring the pH of a solution containing the weak acid at 0.30 M and its conjugate base at 0.30 M. The measured pH was 8.0. What is the pKa of the weak acid?