A 1.250 g sample of cheese was subjected to a Kjeldahl analysis to determine the amount of protein. The sample was digested, the nitrogen is oxidized to NH₄⁺, and was then converted to NH₃ with NaOH, and distilled into a collection flask containing 50.00 mL of 0.1050 M HCl. The excess HCl is back titrated with 0.1175 M NaOH, this required 21.65 mL to reach the bromothymol blue end point. A. How many moles of N is present in the cheese sample? B. Report the %N of the cheese sample. C. Report the %protein of the cheese sample, assuming that there are 6.4 grams of protein for every gram of nitrogen

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A 1.250 g sample of cheese was subjected to a Kjeldahl analysis to determine the amount of protein. The sample was digested, the nitrogen is oxidized to NH₄⁺, and was then converted to NH₃ with NaOH, and distilled into a collection flask containing 50.00 mL of 0.1050 M HCl. The excess HCl is back titrated with 0.1175 M NaOH, this required 21.65 mL to reach the bromothymol blue end point. A. How many moles of N is present in the cheese sample? B. Report the %N of the cheese sample. C. Report the %protein of the cheese sample, assuming that there are 6.4 grams of protein for every gram of nitrogen

 

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