A 100-mL buffer solution with pH of 4.80 is prepared as a stock solution. Using this stock buffer solution, three buffers are prepared by diluting a given volume of the stock buffer (as shown below) to 100 mL with water.
A 100-mL buffer solution with pH of 4.80 is prepared as a stock solution. Using this stock buffer solution, three buffers are prepared by diluting a given volume of the stock buffer (as shown below) to 100 mL with water.
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter19: Biomedical Laboratory Instrumentation
Section: Chapter Questions
Problem 7P
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![4. A 100-mL buffer solution with pH of 4.80 is prepared as a stock solution. Using this stock
buffer solution, three buffers are prepared by diluting a given volume of the stock buffer (as
shown below) to 100 mL with water.
Volume used from
Buffer
Final volume, mL
original solution, mL
1
5
100
2
10
100
20
100
a. Do they have the same pH? Check the box corresponding your answer.
O Yes
O No
HY 47.1 Basic Biochemistry, Laboratory
b. Which is true about their absolute concentrations? Check the box corresponding your
answer.
[Buffer 1] > [Buffer 2] > [Buffer 3]
[Buffer 3] > [Buffer 2] > [Buffer 1]
[Buffer 3] = [Buffer 2] = [Buffer 1]
[Buffer 3] = [Buffer 2] > [Buffer 1]
[Buffer 3] > [Buffer 2] = [Buffer 1]
c. Which is true about their concentration ratios? Check the box corresponding your
answer.
[Buffer 1] > [Buffer 2] > [Buffer 3]
[Buffer 3] > [Buffer 2] > [Buffer 1]
[Buffer 3] = [Buffer 2] = [Buffer 1]
[Buffer 3] = [Buffer 2] > [Buffer 1]
[Buffer 3] > [Buffer 2] = [Buffer 1]
%3!
%3D
d. Which of the following is true about the ability of the three buffers to resist pH change
when 1 mL of HCI is added to each buffer? Check the box corresponding your answer.
Ability to resist pH change: Buffer 1< Buffer 2 < Buffer 3
Ability to resist pH change: Buffer 1 > Buffer 2 > Buffer 3
Ability to resist pH change: Buffer 1 = Buffer 2 = Buffer 3
Ability to resist pH change: Buffer 1 < Buffer 2 = Buffer 3
Ability to resist pH change: Buffer 1 = Buffer 2 < Buffer 3
e. Which solution has the highest buffer capacity? Check the box corresponding your
answer.
Buffer 1
Buffer 2
Buffer 3
Their buffer capacities are equal](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fdd84f37f-4b1c-470e-80c9-940c2e9ca664%2F147eefdf-fbe4-47f4-96e9-70b4c51ef287%2Fic6po7_processed.png&w=3840&q=75)
Transcribed Image Text:4. A 100-mL buffer solution with pH of 4.80 is prepared as a stock solution. Using this stock
buffer solution, three buffers are prepared by diluting a given volume of the stock buffer (as
shown below) to 100 mL with water.
Volume used from
Buffer
Final volume, mL
original solution, mL
1
5
100
2
10
100
20
100
a. Do they have the same pH? Check the box corresponding your answer.
O Yes
O No
HY 47.1 Basic Biochemistry, Laboratory
b. Which is true about their absolute concentrations? Check the box corresponding your
answer.
[Buffer 1] > [Buffer 2] > [Buffer 3]
[Buffer 3] > [Buffer 2] > [Buffer 1]
[Buffer 3] = [Buffer 2] = [Buffer 1]
[Buffer 3] = [Buffer 2] > [Buffer 1]
[Buffer 3] > [Buffer 2] = [Buffer 1]
c. Which is true about their concentration ratios? Check the box corresponding your
answer.
[Buffer 1] > [Buffer 2] > [Buffer 3]
[Buffer 3] > [Buffer 2] > [Buffer 1]
[Buffer 3] = [Buffer 2] = [Buffer 1]
[Buffer 3] = [Buffer 2] > [Buffer 1]
[Buffer 3] > [Buffer 2] = [Buffer 1]
%3!
%3D
d. Which of the following is true about the ability of the three buffers to resist pH change
when 1 mL of HCI is added to each buffer? Check the box corresponding your answer.
Ability to resist pH change: Buffer 1< Buffer 2 < Buffer 3
Ability to resist pH change: Buffer 1 > Buffer 2 > Buffer 3
Ability to resist pH change: Buffer 1 = Buffer 2 = Buffer 3
Ability to resist pH change: Buffer 1 < Buffer 2 = Buffer 3
Ability to resist pH change: Buffer 1 = Buffer 2 < Buffer 3
e. Which solution has the highest buffer capacity? Check the box corresponding your
answer.
Buffer 1
Buffer 2
Buffer 3
Their buffer capacities are equal
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