Kindly answer question a & b The following data were obtained for a set of standards using UV-VISspectrophotometric analysis for the determination of caffeine in tea sample.Caffeine (ppm)Absorbance10.000.07920.000.15930.000.26340.000.34450.000.443(a) Briefly describe the meaning of chromophores.(b) (i)Depict the graph using Microsoft excel(ii)Identify the caffeine concentrations in two unknown samples, X and Y,having absorbance values of 0.123 and 0.376.

(a) Chromophore is a group which exhibit absorption of electromagnetic radiation in UV or Visible…

a) Briefly describe the meaning of chromophores. b) (i) Depict the graph using Microsoft excel (ii) Identify the caffeine concentrations in two unknown samples, X and Y, having absorbance values of 0.123 and 0.376.

a) Briefly describe the meaning of chromophores. b) (i) Depict the graph using Microsoft excel (ii) Identify the caffeine concentrations in two unknown samples, X and Y, having absorbance values of 0.123 and 0.376.

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter34: Miscellaneous Separation Methods

Section: Chapter Questions

Problem 34.2QAP

See similar textbooks

Related questions

Q: If 100 grams of glucose (C6H12O6) was dissolved in a solution, calculate the millimoles of oxygen.

A: Mole can be defined as a standard unit for measuring large quantities of very small entities such as...

Q: Using the 13 rule and Hydrogen deficiency indicator (HDI), determine the molecular formula and propo...

Q: For the following statements about chemical equations, check the boxes that represent true statement...

A: Please find your solution below : Chemical reaction is described by a chemical equation. Chemical eq...

Q: Calculate the standard enthalpy change for the reaction at 25 °C. Standard enthalpy of formation val...

Q: What is the molar concentration of NaOH in a solution that contains 1560 ppm to give 750 ml.( Na=23,...

A: Parts per million (PPM) is a unit of measurement used when expressing a very dilute concentration le...

Q: Question 2 of 20 Which of the following compounds would have the highest boiling point? H,C NH2 A) C...

A: Boiling point: The temperature at which the vapor pressure of a liquid becomes equal to the atmosphe...

Q: 11. The conducting loop in Fig- ure Q25.11 is moving into the region between the magnetic poles show...

A: Concepts and Reasons :The concepts needed to solve this problem are the force of charge in a magneti...

Q: in your own words, explain the browning reaction that occurs in cooking ground pork

A: The correct answer is given below

Q: For each of the following IR spectra, determine whether it agrees with the structure of a ketone, an...

A: General IR absorptions of some functional group : Ketone : a sharp peak at 1715 cm-1(C=O stretch) ...

Q: Part A Place the following elements in order of decreasing atomic size: silicon, nitrogen, helium, p...

A: Atomic radius: The distance between the nucleus and the outermost electron is known as the atomic ra...

Q: 7. What is the name of the amino that has an ɛ amino group on its side chain? What is the one letter...

A: The above question is from amino acids. The amino acids which has amino group in ε- position with re...

Q: 4. What is the osmotic pressure of a 0.0522M solution of C 12H22011 at 55 °C? A. 1.1 atm B. 1.4 atm ...

Q: Ketones and aldehydes react with sodium acetylide (the sodium salt of acetylene) to give alcohols, a...

Q: Given the following data 2 CIF(g)+ O2 (g) Cl, O(g) + F,0(g) 2 CIF3 (9) + 202 (g) → Cl, O(9) + 3F,0(g...

A: The enthalpy change is a state function and the Hess law states that the change of enthalpy in a che...

Q: Calculate the enthalpy of formation of water. State the reactants and the products of the reaction a...

A: Enthalpy of formation of water is heat released when 1 mole of water is formed from its pure natural...

Q: Q3-al Calculate Miller's coefficient for the surface that cuts the crystal's coordinates at the Cart...

Q: 4.) Determine if each of the following is a spontaneous process, a nonspontaneous process, or an equ...

A: The answers are given below

Q: The following compound is treated with excess K2Cr2O7. Provide the name of the product that would fo...

A: K2Cr2O7 is an oxidising agent. It oxidised secondary alcohol into ketone.

Q: 3. What is the boiling point of a 6.95M solution of C12H22011 in water? A. 105.2 C B. 107.0 °C C. 10...

A: Colligative properties: The colligative properties are those properties of solutions that depend on ...

Q: .on three components True O False O Number of isomers of the .Ethane is one True O

A: We will be answering all the questions with proper explanation.

Q: Why is there a need for 2 indicators in the analysis of mixed alkali? How do the two indicators help...

A: For a mixed alkali ( two alkalis having different pH range ) we use 2 indicators.

Q: Multiple choice 1. What type of relationship does boiling point and vapor pressure exhibit? Choices...

A: Colligative Property of solution.,

Q: 7.) Calculate the standard free-energy changes for the following reactions at 25°C: (a) CH4(g) + 202...

Q: potassium and sulfur Express your answers as ions and chemical formula separated by a commas. rubidi...

Q: The hydrogen ion concentration of a solution is 0.00001 moles per liter. What is the hydrogen ion co...

A: Given, The hydrogen ion concentration of a solution is 0.00001 mole/ liter. What is the hydrogen io...

Q: Reaction product ( HCI + .Ethylene) is 1-Chloroehtane True False O Quantum is the amount of .energy ...

A: 1) True 2) True

Q: Determine the molarity of a solution with a volume of 445. mL and 0.590 mol of solute dissolved.

Q: A 31.9 mM (millimolar) solution was made using 0.202 L of a 221 mM solution. What is the final volum...

Q: A 15.3 M solution has a volume of 4.0 L. The volume is tripled by adding water. What is the final mo...

A: M1V1 = M2V2 M = molarity of solution(M) V = volume of solution (L) M * V = number of moles Number o...

Q: A solution is made by dissolving 25.1 g of barium acetate, Ba(CH3CO0)2, in enough water to make exac...

Q: NaBH, NaBH, OH PCC CH,CI,

Q: Question 3 What is the reaction order for the decomposition of p-toluenesulfinic acid? 3 CH3C6H4SO2H...

A: Chemical kinetics can be defined as the branch of chemistry that deals with rates of chemical reacti...

Q: [References] It has been determined that the body can generate 5500 kJ of energy during one hour of ...

Q: An organic chemistry student prepared 110. mL of a cyclohexane-ethanol solution at 24.0 °C by measur...

A: The volume of solute in milliliter present in 100 mL solution is known as the volume percent concent...

Q: A student ran the following reaction in the laboratory at 1180 K: 2502(0) + Oala) 2503(0) When she I...

Q: Part A What volume (in mL) of a 0.150 M HNO3 solution will completely react with 35.7 mL of a 0.108 ...

A: The correct answer is given below

Q: Natural cements only used in specialized mortars. Made from calcintion of clay-rich limestones True ...

A: Cement : It is the binder which is used to bind the two materials together by its property setting a...

Q: In the standardization of a solution of NaOH against 2.508 grams of oxalic acid (H2C2O4•2H2O), 42.53...

A: An acid exactly neutralizes the same number of equivalents of base and vice-versa. milliequivalents ...

Q: (2) ukai A 0.250 M solution of Na2C204 is to be used in a reaction in which the C2042- will be oxidi...

Q: ammonium perchlorate The pH will be less than 7. barium nitrite The pH will be approximately equal t...

A: The solutions which are acidic will have pH less than 7, more than 7 will be basic and having pH 7 w...

Q: Question 1 This following is the reaction for the synthesis of ammonia: N2 + 3 H2 + 2 NH3 At a certa...

Q: 1. How are each of the structures A-F related to structure X? They could be E (enantiomers), D (dias...

Q: It has been dete of energy during

A: Given the amount of heat generated by our body during 1 hour of exercise = 5500 kJ / 1 hour The heat...

Q: subject) - maryhar X M <3- mary.h Ô https://www-awu.aleks.com/alekscgi/x/Isl.exe/1o_u-IgNslkr7j8P3jH...

Q: CH3 HCI CH3 H3C (b) H3C. HBr H3C. CH3 Br (с) HBr or (d) HCI

A: Given

Q: i need the answer quickly

A: Since you asked specific multiple questions so, as per Q&A guidelines of portal I solved all th...

Q: 1. Which will be the major product if 3,3-demethyl-2- butanol to react in 85% of H3P04? a. 2,3-demet...

A: Alcohol gives dehydration reactions when they react with a strong acid like H2SO4 or H3PO4 to form a...

Q: The following compound is treated with 1 equivalent of K2Cr207. Provide the name of the product that...

Q: 38.3 g KNO3 Express your answer in moles to three significant figures. mol KNO3 Submit Request Answe...

A: Given, Mass of potassium nitrate(KNO3) = 38.3 g Number of moles of KNO3 = ?

Q: valence electron

Question

Kindly answer question a & b

The following data were obtained for a set of standards using UV-VIS
spectrophotometric analysis for the determination of caffeine in tea sample.
Caffeine (ppm)
Absorbance
10.00
0.079
20.00
0.159
30.00
0.263
40.00
0.344
50.00
0.443
(a) Briefly describe the meaning of chromophores.
(b) (i)
Depict the graph using Microsoft excel
(ii)
Identify the caffeine concentrations in two unknown samples, X and Y,
having absorbance values of 0.123 and 0.376.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step by step

Solved in 2 steps with 1 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

3.Explainthethin-layerchromatographicpuritycheckprocedureforanalysisofanunknownorganic compound.
what is Optical rotatory dispersion and circulatory dichroism method for protein binding?
1. What is the significance of Rf values?2. What is the basis for the separation of components of mixtures by paperchromatography?3. Give 3 important applications of chromatography.4. Define or describe each of the following terms: - Chromatography- Development- Solvent front- Stationary phase - Chromatogram- Adsorbent- Mobile phase- Rf
Principles of separation by capillary Isolachophoresis
The required mobile phase for the analysis caffeine in soft drinks in liquid chromatography are methanol and water. What class of methanol and water must be used? Kindly explain the answer.
Under what wavelength (in nm) should a spectrophotometer be set to be able to measure the optical density of microbial cultures? What could be viable reference blanks we can use to zero our spectrophotometer when measuring the optical density of microbial cultures?
One common way to determine phosphorous in urine is to treat the sample after removing the protein with molybdenum (VI) and then reducing the resulting 12-molybdophosphate complex with ascorbic acid to give an intense blue-coloured species called molybdenum blue. The absorbance of molybdenum blue can be measured at 650 nm. A 24-hour urine sample was collected, and the patient produced 1122 mL in 24 hours. A 1.00 mL aliquot of the sample was treated with Mo (VI) and ascorbic acid and diluted to a volume of 50.00 mL A calibration curve was prepared by treating 1.00 mL aliquot of phosphate standard solution in the same manner as the urine sample. The absorbances of the standards and the urine sample were obtained at 650 nm and the following results were obtained. ppm, P aBSORBANCE (A) 1.003.00 0.230 2.00 0.436 3.00 0.638 4.00 0.848 Urine sample 0.518 ffind the slope and intercept, then graph it.
Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the demonstration in TLC and paper chromatography? How do you say so?
A student developed a chromatogram and found that the eluent front traveled 69 mm and the Zinc cation traveled 24 mm. In the development of a chromatogram of a mixture of cations, the eluent front traveled 52mm. If the Zinc cation is a cation of the unknown mixture, where will its band appear in the chromatogram?
Briefly explain the principle behind the following techniques:(a) ion-exchange chromatography (b) gel filtration chromatography (c) affinity chromatography (d) Use three diagrams to illustrate the separation of three different proteins by thesemethods.
You are assigned to separate a mixture of colourless amino acids present in protein molecule, using chromatography technique. You have a chrome paper (Filter paper sheet), TLC plate, chrome tank or beaker, Solvent (Hexane) and the mixture of amino acids. Answer the following questions related to your experiment. (a). Which of the chromatography technique (paper or thin layer) will give better separation in this experiment?
Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the thin layer chromatography of plant pigments? How do you say so?