A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight line with the equation ?=0.0182?+0.007 where ? is the corrected absorbance of the solution and ? is quantity of protein in the solution in units of micrograms (μg). Determine the quantity of protein in a solution that has an absorbance of 0.305. A blank solution has an absorbance of 0.060. The answer (Quantity of protein) should be in units of (μg)

Principles of Modern Chemistry
8th Edition
ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Chapter20: Molecular Spectroscopy And Photochemistry
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A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight line with the equation

?=0.0182?+0.007

where ? is the corrected absorbance of the solution and ? is quantity of protein in the solution in units of micrograms (μg). Determine the quantity of protein in a solution that has an absorbance of 0.305. A blank solution has an absorbance of 0.060. The answer (Quantity of protein) should be in units of (μg)

 
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