A chromatography column is used to separate four amino acids: glutamic acid, serine, alanine, and histidine. A cylindrical column is packed (65% by volume) with nonporous polymer particles. An aqueous solution containing 25 mol % of each amino acid is fed into the column with a superficial solution velocity of 0.32 mm/sec) The column is operated continuously using a 550 sec feed (pulse) time followed by elusion for a set period of time. Given the equilibrium constants below for each amino acid, answer the following sets of questions: A. What is the minimum length of the column required to completely separate the amino acids? B. What is the time required for solvent elution (time for all amino acids to exit the column)?
A chromatography column is used to separate four amino acids: glutamic acid, serine, alanine, and histidine. A cylindrical column is packed (65% by volume) with nonporous polymer particles. An aqueous solution containing 25 mol % of each amino acid is fed into the column with a superficial solution velocity of 0.32 mm/sec) The column is operated continuously using a 550 sec feed (pulse) time followed by elusion for a set period of time. Given the equilibrium constants below for each amino acid, answer the following sets of questions: A. What is the minimum length of the column required to completely separate the amino acids? B. What is the time required for solvent elution (time for all amino acids to exit the column)?
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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A chromatography column is used to separate four amino acids: glutamic acid, serine, alanine, and histidine. A cylindrical column is packed (65% by volume) with nonporous polymer particles. An aqueous solution containing 25 mol % of each amino acid is fed into the column with a superficial solution velocity of 0.32 mm/sec) The column is operated continuously using a 550 sec feed (pulse) time followed by elusion for a set period of time. Given the equilibrium constants below for each amino acid, answer the following sets of questions:
A. What is the minimum length of the column required to completely separate the amino acids?
B. What is the time required for solvent elution (time for all amino acids to exit the column)?
Transcribed Image Text:Amino Acid
Glutamic acid, R=(CH₂)2COOH
Serine, R=CH₂OH
Alanine, R-CH3
Histidine, R=CH₂(C3N₂H3)
Kdi
1.52 147.13
2.87
105.09
1.94
89.09
2.40
155.15
Mw (g/mol)
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