A circular DNA molecule is subjected to complete restriction digestion by (1) BamHI alone, (2) Haelll alone, and (3) both enzymes together. The DNA fragments are then separated using gel electrophoresis. Results are shown in Figure 2: BamHI Haelll Both
Q: A circular DNA of 40 kb (40,000 bases) length is cut with a restriction enzyme whose precise…
A: The probability of a restriction enzyme to be found in a DNA is 4n, where n is the number of bases…
Q: The red line indicates where a radioactively labeled probe hybridizes. Three samples of the genomic…
A: P B PB…
Q: Restriction enzymes and DNA ligase play essential roles in DNA cloning. How is it that a bacterium…
A: Deoxyribonucleic acid (DNA) is a particle made out of two polynucleotide chains that loop around one…
Q: A restriction enzyme digests DNA into fragments. Name the technique used to check the progression of…
A: DNA and RNA are nucleic acids that are found in living cells. Almost all living cells contain both…
Q: A new restriction enzyme is discovered. It recognizes a 6 base pair pallindromic sequence. The first…
A: BASIC INFORMATION RESTRICTION ENZYME they are also known as molecular scissors it was in the year…
Q: Which of the restriction enzymes listed in the table below produces blunt-end fragments? Enzyme…
A: The blunt end are produced by the restriction enzymes when the end of a DNA fragment after breaking…
Q: The following plasmid is digested with EcoR1 and Ndel restriction endonucleases. Agarose…
A: Restriction endonucleases are enzymes which cleave the phosphodiester bonds within a DNA. These…
Q: A 3000 bp circular DNA was treated with both HindIII and EcoRI restriction enzymes. EcoRI cuts at…
A: In this question, we are given two restriction enzymes EcoRI and HindIII with their restricting…
Q: Which of the following sequences, when combined with itscomplement, would be clipped by a…
A: Restriction endonuclease is also known as restriction enzymes that are produced by the bacteria.…
Q: Draw a diagram showing what pGEM will look like after it has been digested with BamHI.
A: BamHI: BamHI is a restriction enzyme obtained from Bacillus amyloliquefaciens that specifically cuts…
Q: They have purified a 1100 bp HindIII restriction fragment that they plan to sequence. As a first…
A: EcoR1: EcoR1 is a restriction endonuclease enzyme found in the E. coli bacteria. It's a restriction…
Q: For sample 2, how many different size fragments will be generated after completing digestion with…
A: Introduction Enzymes are the crucial biomolecules which assists in various biochemical reactions…
Q: A plasmid DNA and a linear DNA (both of the same size) have one site for a restriction endonuclease.…
A: Because plasmid is a circular DNA molecule, this is the case. It becomes linear but not fragmented…
Q: A molecule of double-stranded DNA that is 5 million base pairs long has a base composition that is…
A: If a molecule of double-stranded DNA which is 5 million base pairs long, has a base composition that…
Q: There are three classes of restriction enzymes; Class I, Class II and Class II. Nevertheless, Class…
A: Restriction endonuclease are the enzyme which make a cut in genetic material that is DNA somewhere…
Q: Can DNA be isolated from beef? Discuss the process briefly
A: The purification of DNA from the collected sample of DNA with the help of the various methods and…
Q: Restriction Enzymes with LongerRecognition Sites Produce Larger _______ Fragments
A: The restriction enzyme is the molecular scissors that cut at a specific site. These are naturally…
Q: Consider a partial restriction digestion, in which genomic DNA is exposed to a small, limiting…
A: The process of cutting or cleaving DNA by using restriction enzymes is called digestion or…
Q: 2. Your Genetics professor gives you and your classmate's information on a restriction digest. The…
A: The advantages of biotechnology for our society can be in drug manufacturing at a low cost using…
Q: The following double stranded DNA fragment was double digested with BamHI and Kpnl. If you ran the…
A: Restriction enzymes are the enzymes which cut the DNA at specific sequences. The specific sequence…
Q: Which restriction enzyme used in your simulated electrophoresis experiment produced DNA with ‘sticky…
A: Restriction Enzymes or "Restriction endonucleases" are proteins that cut DNA at a specific base…
Q: A purified piece of DNA of 1650bp is cut with Hind III and Pst I separately and then with both…
A: Restriction enzymes are endonucleases derived from bacteria that make a double-stranded cut in the…
Q: Identify the basic steps (in right order) in AFLP marker analysis. Explain briefly how this marker…
A: Basic steps in AFLP marker analysis.
Q: If a uidA amplicon generateed by PCR is 200bp and the DNA fragments resulting from the restriction…
A: INTRODUCTION Polymerase Chain reaction This is the method of amplification of of nucleic acids such…
Q: Consider a partial restriction digestion, in which genomic DNA is exposed to a small, limiting…
A: The process of cutting or cleaving DNA by using restriction enzymes is called digestion or…
Q: Calculate the average sizes and numbers of DNAfragments produced by digesting human genomic DNAwith…
A: DNA is the genetic material of many eukaryotic and prokaryotic organisms. It is a double-stranded…
Q: Dr. Doom has a DNA sequence of 2000 bp. Enzyme X and Enzyme Y restriction sites and their positions…
A: Restriction enzymes are the enzymes which cut DNA at particular site.
Q: molecular scissors", justify that
A: Restriction enzymes are found in a wide variety of prokaryotes. Restriction enzymes received their…
Q: When using blunt-end primers, how would you determine the correct plasmid construct from the…
A: Ligation is the joining or ligation of two nucleic acid fragments through the action of a ligase…
Q: State the characteristics that would be required for using a restriction endonuclease with human…
A: Restriction enzymes or restriction endonuclease are the enzyme naturally present in the bacteria…
Q: restriction enzyme
A:
Q: A DNA fingerprint consists of sections of DNA that have been cut with restriction endonuclease…
A: DNA fingerprinting DNA fingerprinting is a modern technique that help us to solve crimes. The…
Q: A DNA probe with sequence TCAGGCTTCAG would bind most strongly to which of the following DNA…
A: DNA probes are single-stranded DNA segments that are hybridised to indicate the existence of…
Q: Write the sequences of the two 12-residue primers that could be used to amplify the following DNA…
A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…
Q: If the Hinfl restriction enzyme recognizes G^ACTC sites, as seen in the image, belUW. GACTC How many…
A: The cloning of DNA is the mechanism by which a certain fragment of DNA is multiplied. The selected…
Q: To terminate a reaction in which a restriction enzyme cleaves DNA, researchers often add high…
A: Restriction enzymes are endonucleases that identify and cleave at specific sequences within DNA.…
Q: The partial sequence of one strand of a double-stranded DNA molecule is5′ – – –…
A: Restriction enzymes also called molecular scissors are originally a part of a bacterial defence…
Q: Restriction sites are palindromic; that is, they read the same in the5' to 3' direction on each…
A: Restriction enzymes also called molecular scissors are originally a part of a bacterial defence…
Q: Consider the DNA segment with a sequence: 3'-TACGGTACGGGATTG-5'. If the given DNA sample was…
A: Pyrosequencing is a form of sequencing that works by the principle of DNA replication. The template…
Q: Suppose that a geneticist discovers a new restriction enzyme in the bacterium Aeromonas ranidae.…
A: Restriction enzymes are found in bacteria, their function is to recognise and cut a specific site on…
Q: A 10 kb DNA fragment digested with the restriction endonuclease EcoRI yields fragments of 4 kb and 6…
A: Restriction enzymes are also known as restriction endonucleases. These are the enzymes produced by…
Q: Restriction endonuclease and ligase are two types of enzymes used in the process of genetic…
A: DNA stands for deoxyribonucleic acid. It is the genetic material of the organisms that transfer from…
Q: sequence? Which enzyme will cut this sequence and where in the sequence? What is the sequence of the…
A: Given: Need to find the recognition sequence and sequence of the 'sticky end' produced by this…
Q: triction enzyme digests DNA into fragments.term the technique used to check the progression of this…
A: Living cells include nucleic acids such as DNA and RNA. Almost all live cells have both RNA and DNA,…
Q: Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting…
A: Restriction enzymes are also known as molecular scissors because they cut the DNA at specific, short…
Q: A small DNA molecule was cleaved with several different restriction nucle- ases, and the size of…
A: Introduction: DNA is a type of nucleic acid that is present in the nucleus of the cell. It is the…
Q: The double stranded DNA sequence of a restriction enzyme cut site is typically complementary,…
A: Restriction enzymes are those enzymes which cut DNA by recognizing target sequences. Restriction…
Q: Restriction enzymes are used in laboratory manipulations to cut DNA; many of these enzymes can be…
A: Restriction enzyme - Restriction enzyme are also called as Restriction endonuclease. restriction…
Q: Explain how restriction enzymes recognize and digest DNA to create either blunt or sticky ends
A: Answer: Introduction: Restriction endonucleases enzymes which cut DNA at precise recognition sites.…
ii
Step by step
Solved in 2 steps
- If the GAATTC palindrome repeats are randomly found along the DNA strand, then what can you say about the sizes of the fragments that will be produced when the DNA is digested with a restriction enzyme that recognizes that sequence? How does the total length of the fragments relate to the size of the original DNA fragment?When circular DNA is sequenced, the nucleotide base pairs are numbered starting from a fixed position on the DNA, all the way around, usually in a clockwise manner. a DNA molecule that is 3133 base pairs long is digested with RsaI restriction enzyme recognition sites at base numbers 366, 1534, and 2207. What are the sizes of the DNA fragments that will be produced after the DNA is digested with RsaI?A linear piece of DNA that is 30 kb long is first cut with BamHI, then with HpaII, and, finally, with both BamHI and HpaII together. Fragments of the following sizes were obtained from this reaction: BamHI: 20-kb, 6-kb, and 4-kb fragments Hpall: 21-kb and 9-kb fragments BamHI and Hpall: 20-kb, 5-kb, 4-kb, and 1-kb fragments Draw a restriction map of the 30-kb piece of DNA, indicating the locations of the BamHI and HpaII restriction sites
- Consider the ends of the DNA fragments shown below. They have been produced by digestion of a single sequence of DNA using a number of restriction endonucleases. 1. 5'A 3' 3'TTCGA5' 2. 5'G 3' 3'CAGCT5' 3. 5'AATTC3' 3' G5 4. 5'TCGAC3' 3' G5' 5. 5'GGG 3' 3'CCC 5' Which of these ends are capable of annealing and being joined by DNA ligase?How would the results of this activity have been different if the DNA sequence you digested were circular instead of linear? When restriction digests are performed and analyzed in the laboratory, one lane of the agarose gel is loaded with DNA ladders, which are DNA fragments of known sizes. What is the point of including these size standards?When linear DNA is sequenced, the nucleotide base pairs are numbered from the start to finish. a DNA molecule that is 3133 base pairs long is digested with RsaI restriction enzyme recognition sites at base numbers 366, 1534, and 2207. What are the sizes of the DNA fragments that will be produced after the DNA is digested with RsaI?
- The restriction enzyme Alu I cleaves at the sequence 5’-AGCT- 3' , and Not I cleaves at 5' -GCGGCCGC-3'. What would be the average distance between cleavage sites for each enzyme on digestion of double-stranded DNA? Assume that the DNA contains equal proportions of A, G, C, and T.When joining two or more DNA fragments, a researcher can adjust the sequence at the junction in a variety of subtle ways, as seen in the following exercises.(a) Draw the structure of each end of a linear DNA fragment produced by an EcoRI restriction digest (include those sequences remaining from the EcoRI recognition sequence).(b) Draw the structure resulting from the reaction of this end sequence with DNA polymerase I and the four deoxynucleoside triphosphates.(c) Draw the sequence produced at the junction that arises if two ends with the structure derived in (b) are ligated (d) Draw the structure produced if the structure derived in (a) is treated with a nuclease that degrades only single-stranded DNA.(e) Draw the sequence of the junction produced if an end with structure (b) is ligated to an end with structure (d).(f) Draw the structure of the end of a linear DNA fragment that was produced by a PvuII restriction digest (include those sequences remaining from the PvuII recognition…Restriction endonuclease digestion of a DNA sequence yielded fragments of the following sizes: 1. 5.2 kb 2. 0.8 kb 3. 1.2 kb 4. 3.8 kb 5. 3.1 kb After gel electrophoresis, what would be the order in which these fragments would be found—the last fragment listed being furthest from the negative pole.
- If a restriction enzyme cuts between the G and the A whenever itencounters the sequence GAATTC, how many fragments will beproduced when the enzyme is digested with DNA with the followingsequence? TGAGAATTCAACTGAATTCAAATTCGAATTCTTAGCa. Two c. Fourb. Three d. FiveA DNA probe with sequence TCAGGCTTCAG would bind most strongly to which of the following DNA fragments? a. AGTCCGAAGTC c. GACTTCGGACT b. TCAGGCTTCAG d. UGAGGCUUGAGA 22-kb piece of DNA has the following restriction sites:A batch of this DNA is first fully digested by HpaI alone, then another batch is fully digested by HindIII alone, and finally, a third batch is fully digested by both HpaI and HindIII together. The fragments resulting from each of the three digestions are placed in separate wells of an agarose gel, separated by gel electrophoresis, and stained by ethidium bromide. Draw the bands as they would appear on the gel.