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Name: The following DNA sequence contains a six-base sequence that is a reco
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Description: The following DNA sequence contains a six-base sequence that is a recognition and cutting site for a bacterial restriction enzyme. What is the recognition sequence? Which enzyme will cut this sequence and where in the sequence? What is the sequence o

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sequence? Which enzyme will cut this sequence and where in the sequence? What is the sequence of the 'sticky end' produced by this enzyme? 5’ CCGAGAAGCTTAC 3’

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 7QP: Cloning Genes Is a Multistep Process The following DNA sequence contains a six-base sequence that is...

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Cloning Genes Is a Multistep Process The following DNA sequence contains a six-base sequence that is a recognition and cutting site for a restriction enzyme. What is this sequence? Which enzyme will cut this sequence? (See Figure 13.5 for help.) 5 CCGAGGAAGCTTAC 3 3 GGCTCCTTCGAATG 5
Which of the following is a sequence most likely to be recognized by restriction enzymes? a. 5' GATTAATC 3' - double-strand form b. 5' ATG 3' - double-strand form c. 5' AUGCGCAU 3' - double-strand form d. NH3-met-trp-val- COOH e. none of the above
Which of the following sequences, in the double-stranded form, can probably be recognized by a restriction enzyme? Choose an answer from below? TTCCTT CGACGA GTCGAC GGTTGG GAGGAC
Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme? AAGG TTCC GGCC CCGG ACCA TGGT AAAA TTTT
Which of the following sequences in double-stranded DNA ismost likely to be recognized as a cutting site for a restrictionenzyme?(A) AAGGTTCC(B) GGCCCCGG(C) ACCATGGT(D) AAAATTTT
Which of the following is true about restriction endonucleases?a) Type I and II requires ATP to move along DNAb) Type I, II and III requires ATP to move along DNAc) Type II requires no ATP and cleaves DNA within recognition sequenced) Type II requires ATP and cleaves DNA within recognition sequence
A new restriction enzyme is discovered. It recognizes a 6 base pair pallindromic sequence. The first three bases of the target sequence are are given below with the cut identified by ^. Answer the following questions: Target sequence: 5' G^TA _ _ _ 3'3' _ _ _ _ _ _ 5' 1) Complete the target sequence with all missing bases. Also include the position of the cut in the lower sequence(^) 2) What kind of clevage does the enzyme use?
For the DNA sequence shown, indicate the products of its cleavage with the following restriction endonucleases (AKA restriction enzymes):5′-ACAGCTGATTCGAATTCACGTT-3′3′-TGTCGACTAAGCTTAAGTGCAA-5′a) EcoRI (the recognition sequence and cleavage site is G↓AATTC);b) AluI (the recognition sequence and cleavage site is AG↓CT).
A molecule of double-stranded DNA that is 5 million base pairs long has a base composition that is 62% G + C. How many times, on average, are restriction sites for the following restriction enzymes likely to be present in this DNA molecule? a. BamHI (recognition sequence is GGATCC)
A molecule of double-stranded DNA that is 5 million base pairs long has a base composition that is 62% G + C. How many times, on average, are restriction sites for the following restriction enzymes likely to be present in this DNA molecule? a. HindIII (recognition sequence is AAGCTT)
Which of the following is a sequence at which a restriction enzyme would cut? A) 5'AAGCAA3' B) 5'AAGCTT3' C) 5'ACAACA3' D) 5'AGAGAG3' E) 5'AGCCGT3' F) 5'AATTAAC3'
An 800 bp fragment is completely digested with a single restriction enzyme. Fragments of 50*, 150*, 200, and 400 bp are found. (Asterisks denote radioactively labeled end pieces.) Which order is consistent with the results? Group of answer choices 150, 200, 50, 400 150, 400, 50, 200 150, 200, 400, 50 400, 150, 50, 200