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- QUESTION 24 During lagging strand synthesis of DNA, Okazaki fragments are linked together by ___________. DNA polymerase I Primase Beta clamps DNA LigaseQuestion 36 Which of these is not considered a method of DNA extraction? organic filtration differential purification all of the above are extraction methodsQUESTION 25 During lagging strand synthesis of DNA, the RNA primers are replaced with DNA by __________. DNA Polymerase I DNA Polymerase II DNA Polymerase III Primase
- Question 2. Retroviruses are used in gene therapy. The goal of gene therapy is to insert in the patient genome a copy of a functional gene that is defective in the patient. Since Retroviruses integrates their genome into the host genome they are ideal gene therapy delivery systems. What would be a potential risk of this type of treatment? The individual treated could be more susceptible to infection by other retroviruses Insertion of the retroviral genome into the host genome can cause dangerous mutations. There are not recognized risks with this gene therapy approach. Genes from the host can be inserted into the retroviruses and laterally moved to other cells.Question Number 3: Write the sequence of the mRNA molecule synthesized from a DNA template coding strand having the sequence 5’ – ATCGTACCGTTA – 3QUESTION NO. 1 A transition mutation A. occurs when a purine is substituted for a pyrimidine or vice versa. B. results from the insertion of one or two bases into the DNA chain. C. is most frequently caused by chemicals (like acridine) that intercalate into DNA. D. results from substitution of one purine for another or of one pyrimidine for another. E. always is a missense mutation QUESTION NO. 2 Degeneracy of the generic code denotes the existence of A. multiple codons for a single amino acid. B. codons consisting of only two bases. C. base triplets that do not code for any amino acid. D. different systems in which a given trip let codes for different amino acids. E. codons that include one or more of the unusual bases. QUESTION NO. 3 Replication A. requires that a phosphodiester bond of the incoming dNTP be hydrolyzed in order to be added to the growing chain. B. uses 5' to 3' polymerase activity to synthesize one…
- Question 44 Along with modification of histones and positioning of nucleosomes, following is one of the main epigenetic mechanisms operating across the genome: Question 44 options: DNA acetylation DNA methylation DNA oxidation DNA phosphorylation Question 45 Changes in our DNA that have not been corrected by cellular DNA repair systems create: Question 45 options: Transcripts DNA variants Polypeptide Polynucleotide Question 46 The degeneracy of the genetic code most often involves: Question 46 options: the third base of the codon the second base of the codon the first base of the codon two bases of the codonQUESTION NO. 1 Antisense nucleic acids A. complementary to mRNA would enhance translation . B. could result if a gene is inserted downstream of a promoter but in opposite direction to normal. C. can have no clinical uses. D. react only with DNA. E. are necessary for recombinant DNA technology. QUESTION NO. 2 There is a window in which the effect is primarily on viral replication since AZT is much less effective at competing with dTTP for incorporation by cellular DNA polymerases because of the proofreading ability of DNA polymerases. Proofreading activity co maintain the fidelity of DNA synthesis A. occurs after the synthesis has been completed. B. is a function of 3' co 5' exonuclease activity intrinsic to or associated with DNA polymerases. C. requires the presence of an enzyme separate from the DNA polymerases. D. removes mismatched bases in the interior of the chain. E. does nor occur in prokaryotes.QUESTION NO. 1 Antisense nucleic acids A. complementary to mRNA would enhance translation . B. could result if a gene is inserted downstream of a promoter but in opposite direction to normal. C. can have no clinical uses. D. react only with DNA. E. are necessary for recombinant DNA technology.QUESTION NO. 2 There is a window in which the effect is primarily on viral replication since AZT is much less effective at competing with dTTP for incorporation by cellular DNA polymerases because of the proofreading ability of DNA polymerases. Proofreading activity co maintain the fidelity of DNA synthesis A. occurs after the synthesis has been completed. B. is a function of 3' co 5' exonuclease activity intrinsic to or associated with DNA polymerases. C. requires the presence of an enzyme separate from the DNA polymerases. D. removes mismatched bases in the interior of the chain. E. does nor occur in prokaryotes. QUESTION NO. 3…
- Question 1. Although we will not be doing a gel electrophoresis, data from a gel digest of a Bacillus anthrax plasmid is provided so you can do a DNA map. The Bacillus anthrax plasmid is 4000bp (4Kb) long. Note the origin position as well as the reference molecular weight markers on the gel. Two restriction enzymes, A and B, were used to obtain two individual digests, A and B. They were combined to produce the third digest. The restriction enzyme fragment pattern for the digest of Bacillus anthrax plasmid Determining the Number of Fragments How many fragments were produced by enzyme A? How many fragments were produced by enzyme B? How many fragments were produced by the combined digest (A and B)? Fragment Size Fragment size is relative to molecular weight, and must be determined by comparing the fragment distance to the molecular weight markers. The fragment size has been provided on the gel pattern for this exercise. To make a map you must determine the relative positions of the…Question 41 Redundancy in the genetic code means: Question 41 options: that many base substitutions in coding DNA are synonymous (silent) and do not affect the protein sequence that DNA sequence variations always affect the protein sequence DNA sequence is redundant protein translation is independent of the genetic code Question 42 The mismatch repair (MMR) components work closely with the DNA replication machinery. Question 42 options: True False Question 43 Following could be classified as mutagens except: Question 43 options: Non-ionizing ultraviolet radiation X-rays, gamma rays White light Harmful chemicalsQUESTION 11.1 Give an overview of the possible applications of microbial biotechnology that can be used to curb the spread of newly emerging diseases such as Covid-19. 1.2 Although recombinant DNA technology has proven beneficial in health, agriculture and the environment, its public perception has always been an issue. In your OWN views, discussthe possible social and ethical implications that could make the public hesitant to accept some products of recombinant DNA technology