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- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymeraseWhat type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with the amount of PCRproduct.
- In which stage of genetic engineering a probe is used?a) Cleaving DNAb) Recombining DNAc) Cloningd) ScreeningDuring dna electrophoresis.....fragments migrate toward the..... electrode fasterthan .....fragments. small/large,positive or negative,large or smallWhat molecular biology technique is used to amplify DNA from an evidence sample? Reverse dot blotting Southern hybridization Cloning Polymerase chain reaction (PCR) Capillary electrophoresis
- RECOMBINANT DNA BRIEFLY, DESCRIBE RECOMBINANT DNA AND GIVE ONE CONCRETE EXAMPLE. EVALUATE THE SIGNIFICANCE/PRACTICAL APPLICATIONS OF THIS DNA TECHNOLOGY BY CONSIDERING ETHICAL AND MORAL IMPLICATIONS BEHIND IT. RECOMBINANT DNA EXAMPLE: MODIFIED TRAIT GENE MODIFICATION RECIPIENT ORGANISM FIELDS OF APPLICATION ALSO, WHAT ARE YOUR INSIGHTS? IN 2 TO 3 SENTENCES.Briefly explain the functions of the four procedures you learned about in this lab: DNA extraction PCR Gel electrophoresis DNA sequencing and analysisWhich of the following does NOT require a primer? A) PCR-based viral test B) cloning a human gene from mRNA C) subcloning a bacterial gene from one plasmid to another D) DNA fingerprinting for paternity testing E) DNA sequencing
- What is the relationship between fluorescence intensity of a spot and the amount of DNA in a sample for a Virochip DNA microassay? From the following which is the best choice Less DNA results in greater fluorescene since the laser passes through he sample eaiser More DNA equals to more intense fluorescence since the DNA makes a layer to refelct the laser light More DNA equals to more intense fluorescence since of more dye on the DNA is fluorescene More DNA results in to more fluorescence since there's more DNA to be excted by the laser None of the aboveFluorescently-labeled dideoxynucleotides are used in _____. Group of answer choices:" cDNA libraries DNA sequencing genomic DNA libraries PCRExplain briefly within 5 sentences each 1. a. What is PCR? Explain/relate its importance in DNA marker analysis. 1.b. What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.