A DNA solution of unknown concentration was subjected to quantitative analysis. For better accuracy, 1.25 µL of the DNA solution was mixed with 11.25 µL of buffer wherein, 0.25 µL of this solution was further diluted to a total volume of 2 µL. The final solution was then placed in a nanodrop plate and yielded an absorbance of 0.576 at 260 nm. What is the concentration of DNA in the original solution if the buffer resulted to an absorbance of 0.345 at the same wavelength? 924 ug/mL 739 µg/mL 1342 ug/mL 637 µg/mL

Q: Why does heat denature, or melt, DNA in solution?

A: DNA or deoxyribonucleic acid is the genetic material of most of the organisms except RNA viruses. It…

Q: 4. You isolate DNA from 1 ml of a suspension of E. coli cells using the procedure outlined in your…

A: a. The dilution is 10:1 b. The original DNA concentration (without dilution) is 4400 ug/ml OR 4.4…

Q: In order to determine the purity of a DNA sample. spectrophotometry can be carried out at…

A: DNA absorbs the UV light at an absorbance of 260nm of the wavelength.

Q: A cell sample suspended in a buffer solution was centrifuge at 12,000 rpm. Which of the following…

A: Centrifugation is a frequently used techniques in the cell and molecular biology laboratory. It is a…

Q: What is the purpose of the ethidum bromide that you add to the gel? Why do you need to be careful…

A: Gel electrophoresis Gel electrophoresis, it is a technique by which one can separate and analyse the…

Q: In standard agarose gels used for the analysis of DNA, linear DNA fragments are separated on the…

A: Agarose gel electrophoresis is the most effective, easy, and efficient way to purify, separate,…

Q: In DNA extraction what else might be in the ethanol/aqueous interface? How could you eliminate it

A: Monovalent cations and ethanol play a key role in removing the solvation shell that surrounds DNA,…

Q: If DNA concentration is 2 grams, what would be the molar concentration of human DNA in a human cell?

A: Molar concentration is sometimes referred to as molarity, concentrations of an amount, or content of…

Q: A facility says they need 15 μL of a 40 ng/μL solution of plasmid DNA for sequencing. The typical…

A: Appropriate amount of DNA is one the key elements in gene sequencing. Amount of DNA for sequencing…

Q: How would describe the observation of DNA precipitated out of the water-ethanol solution

A: Ethanol precipitation is a process where ethanol is used as an anti-solvent to purify and…

Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…

A: DNA (Deoxyribonucleic acid) and RNA are two examples of nucleic acids (Ribonucleic acid).…

Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…

A: Given values:  Total DNA extracted = 500 μL Total weight of wheat used for DNA extraction = 200 mg…

Q: onsidering the number of base pairs, compute for the actual length (in micrometers) of this DN

A: There are 7 base pairs. Length of one base pair is 3.4 Angstrom. Length of  7 base pairs is 3.4 X 7=…

Q: what type of gel(in terms of material) can be more suitable for the electrophoresis of 500-1000bp…

A: DNA tests are stacked into spaces toward one side of a gel, and an electric current is applied to…

Q: The relative proportions of cytosine-guanine and adeninethymine bonds in a DNA sample can be…

A: Deoxyribonucleic acid (DNA) is the hereditary material that undergoes replication which is vital for…

Q: Is it possible to estimate concentration of specific DNA sequence in a solution of mixed DNA

A: We can determine the concentration of DNA sequence by these following methods:- 1. Absorbance method…

Q: What is the source of agarose, the powder used to make the gel?                        What is the…

A: Gel electrophoresis is a technique used by scientists to separate DNA or protein molecules.  Gel…

Q: In order to determine the purity of a DNA sample, spectrophotometry can be carried our at Answer to…

A: Spectrophotometry is the quantitative analysis of spectra to compare the relative absorption or…

Q: Identify the CORRECT sequence of processes that takes place during DNA extraction. - ethanol…

A: Introduction : DNA: DNA Extraction Is A Method For Isolating DNA From A Biological Sample.…

Q: When working with the gel electrophoresis chamber, what must we keep in mind? A. Keeping the gel…

A: Gel electrophoresis technique of separation of molecules on the basis of their size and charge under…

Q: IT IS POSSIBLE TO ESTIMATE THE CONCENTRATION OF SPECIFIC DNA SEQUENCE IN A SOLUTION OF MIXED DNA.…

A: DNA can be quantified through nanodrop with a particular absorbance.

Q: Agarose gels with different average pore sizes areneeded to separate DNA molecules of different…

A: Gel electrophoresis is a procedure used in molecular biology that is used for separation of…

Q: In which reagent is extracted DNA suspended to put it in solution? A. Sodium citrate saline buffer…

A: In which reagent is extracted DNA suspended to put it in solution?   A. Sodium citrate saline…

Q: Explain the rationale behind the separation of different cellular components using centrifuge.

A: centrifugation works on the basis of sedimentation rate and density of the cellular components.…

Q: Chargaff’s results yielded a molar ratio of 1.29 for A to G in ox DNA, 1.43 for T to C, 1.04 for A…

A: DNA (deoxyribonucleic acid) is the genetic material of an organism. The DNA is passed on from the…

Q: what happens to the intactness of DNA if extracted DNA fibers were placed in buffer of pH 3?

A: Extracted DNA is generally stored in neutral pH.

Q: If you have G-C rich DNA versus A-T rich DNA, a. will the melting temperature be the same? Why b.…

A: DNA or deoxyribonucleic acid is defined as the molecule that will consist of the organism's genetic…

Q: The Tm of a DNA strand can be calculated by hand using the formula:           (2 ℃)(?????? ?? ? +…

A: Two standard approximation calculations are used. For sequences less than fourteen nucleotides the…

Q: ARE THEY TRUE OR FALSE? a) In a caesium chloride density gradient centrifugation method performed…

A: DNA is the genetic material that helps to control all the activity of the cell. The replication,…

Q: A circular, double-stranded DNA contains 2100 base pairs. The solution conditions are such that DNA…

A: Linking number is the topological property of . Linking number is a total of twists and writhes. The…

Q: What will be the Tm of DNA that has cytosine plus guanine content of 30% compared to that of DNA…

A: The temperature of melting (Tm) is defined as the temperature at which 50% of double stranded DNA is…

Q: Assume complete digestion of a single linear piece of DNA digested (cut) with EcoRI and Hindill.…

A: A plasmid is a single-stranded, circular DNA molecule that is different from the chromosomal DNA of…

Q: 3. You isolate DNA from 1 ml of a suspension of E. coli cells using the procedure outlined in your…

A: a. 10 μl DNA solution was added to 490 μl buffer to make a total volume of 500 μl. Therefore the…

Q: You have isolated genomic DNA and plasmid DNA from a bacterial culture. Please answer the following…

A: Genomic DNA and plasmid DNA are isolated using silica resin columns than using traditional…

Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…

A: Given wheat 200 milligram germ extraction volume. = 500 microlitre Sample used for analysis=…

Q: solution containing single stranded DNA with the sequence 5’ATGGTGCACCTGACTCCTGAGGAGAAGTCTNNNNN’3…

A: In this reaction, we have used dideoxyadenosine triphosphate. Hence, the reaction will stop at all…

Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…

A: DNA generally stands for Deoxyribonucleic acid It is generally double standard Purine and…

Q: Why evenly spaced sequence in chromatogram is an indication of a good DNA chromatogram?

A: The characteristics of an evenly spaced sequence are:-  Evenly-spaced peak, each being…

Q: Consider the DNA segment with a sequence: 3'-TACGGTACGGGATTG-5'. If the given DNA sample was…

A: Pyrosequencing is a form of sequencing that works by the principle of DNA replication. The template…

Q: Consider the four extraction/purification methods .  a. Which extraction method would you use for a…

A: DNA isolated from various biological samples can be used for a vast array of downstream…

Q: A 22-kb piece of DNA has the following restriction sites:A batch of this DNA is first fully digested…

A: The restriction enzymes clean the nucleotide fragment at specific sides called the restriction…

Q: You are given a tube containing 1.5 ml of a DNA solution. The concentration of the DNA solution is…

A: The nucleus of a cell contains the nucleic acids which are responsible for the passing down of…

Q: In DNA isolation techniques, a washing step is always done prior to the final resuspension. What is…

A: DNA isolation: The process of extraction of DNA may be broken down into five primary phases that…

Q: We use 3.16 fold dilution why this dilution schedule is important to determine unknown DNA…

A: The dilutions are the geometric series i.e Serial dilutions are prepared using same dilution step as…

Q: What volume of 4X loading buffer must be added to 21 micro L of DNA in a technique for DNA sample…

A: Electrophoresis is the technique of seperation of RNA DNA and protein molecules on the basis of…

Q: n a pUC19 digest for 1 ug of pUC19 (DNA conc. 282ng/ul) using 10X Cutsmart buffer, pure water and…

A: Restriction digestion is a process by which restriction endonuclease (eg. BamHI) cuts the DNA at a…

Q: Kornberg and his colleagues incubated soluble extracts of E. coli with a mixture of dATP, dTTP,…

A: Radioactivity is the spontaneous emission of energy and subatomic particles from certain kinds of…

Q: Following agarose gel electrophoresis, where on the gel will the largest DNA molecules be, and why?…

A: in agarose gel electrophoresis the agarose gel is poured on the electrophoresis plate and wells are…

Q: DNA of different densities is distinguished by a procedure called caesium chloride (CsCI) density…

A: Cesium chloride (CsCI) density centrifugation generates a gradient of cesium and chloride ions.…

Learning and memory

Learning is defined as a relatively permanent and irreversible adaptive change in behavior. It always results from experience. The ability of an organism to store and recall the effects, sensations, and knowledge gained through learning and experience is called memory.