In order to determine the purity of a DNA sample. spectrophotometry can be carried out at calculated, and a number between to measure DNA, and to measure protein. The ratio is then indicates higher purity. 280 nm; 260 nm; A280/A260; 0.5-1 260 nm; 280 nm; A260/A280; 0.5-1 260 nm; 280 nm; A280/A260; 1.5-2 260 nm; 280 nm; A260/A280; 1.5-2

In order to determine the purity of a DNA sample. spectrophotometry can be carried out at calculated, and a number between to measure DNA, and to measure protein. The ratio is then indicates higher purity. 280 nm; 260 nm; A280/A260; 0.5-1 260 nm; 280 nm; A260/A280; 0.5-1 260 nm; 280 nm; A280/A260; 1.5-2 260 nm; 280 nm; A260/A280; 1.5-2

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter8: The Structure, Replication, And Chromosomal Organization Of Dna

Section: Chapter Questions

Problem 8QP: In analyzing the base composition of a DNA sample, a student loses the information on pyrimidine...

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In order to determine the purity of a DNA sample. spectrophotometry can be carried out at _______ to measure DNA, and _______ to measure protein. The ratio _______ is then calculated, and a number between _______ indicates higher purity. 280 nm; 260 nm; A280/A260; 0.5-1 260 nm; 280 nm; A260/A280; 0.5-1 260 nm; 280 nm; A260/A280; 1.5-2 260 nm; 280 nm; A280/A260; 1.5-2
Which of the following best describes the process of DNA seqencing. a. DNA is seperated on a gel and the different bands are labled with flouroscent nucleotides and scanned with a laser. b. A laser is used to flurorescently label the nucleotides present with in the DNA , the DNA is run on a gel and then the DNA is droken into fragments c. Nucleotides are scanned with a laser and incrprorated into the DNA that has been seperated on a gel and then DNA is amplified with PCR. d. fragments of DNA are produced in a reaction that lables them with any of four different fluroscent dyes and the fragmented then are run on a gel and scanned with laser e. DNA is broken down into its constituents nucleotides and the nucleotides are then run on a gel and purified with a laser
What does the 260 nm light detect when measuring the purity of a DNA sample? Please select the single answer that is most correct. DNA RNA Proteins The sugar-phosphate backbone of the DNA The heterocyclic rings of the DNA
These are the questions choices are given below (i) What is the purpose of adding table salt (NaCl) to the DNA extraction buffer (ii)If ______ alcohol is added to water, the two solutions are ______, forming a ______solution. If ______ alcohol is added to a salted water solution, the two solutions become ______, forming a ______ solution
Describe the function of the following reagents used in the DNA extraction procedure?a) Proteinase K b) 5M Nacl c) Isopropanol d) 1X TE Buffer
The concentration of digested and cleaned DNA is found to be 5μg/mL. How much of your DNA sample (in μL) would require to obtain a final concentration of 30ng.
Watch the demonstration video on nucleic acid quantification on the NanoDrop spectrophotometer: https://cutt.ly/bio150dnaquantification Note: the video is entitled RNA but the method is identical for DNA quantification. 1. At what ratio of A260/280 can we say that DNA is pure? What about RNA and protein?2. While spectrophotometric methods are effective at detecting DNA, a more sensitive but expensive technique called fluorometry is used in sensitive applications. What is the principle behind fluorometry and why is it better than spectrophotometry in detecting DNA?
This piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel.
In general, which part of a DNA sequence will have the best quality? (Ex. Beginning, middle, end?) Why?
The concentration of a DNA sample is 40 nano grams per milliliter how many nanoliters will be needed to obtain 0.5 nano grams of DNA
In analyzing the base composition of a DNA sample, a student loses the information on pyrimidine content. The purine content is A = 27% and G = 23%. Using Chargaff’s rule, reconstruct the missing data and list the base composition of the DNA sample. (Include a brief explanation of how you got your results).
You have a 20 mg/ml of Ethidium bromide stock solution. You need to a final concentration of 2ug/ml into a Agarose solution to visualize DNA. What is the dilution factor? Give typing answer with explanation and conclusion