A cell sample suspended in a buffer solution was centrifuge at 12,000 rpm. Which of the following separation is most likely possible?* A. separation of the cells and the buffer solution B. separation of the DNA C. separation of the cell membrane D. all of the above
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- Which of the following is true? a. In one technique, the DNA sequence can be determined using one strand of DNA as it passes through a nanopore. b. Next generation sequencing is slow but accurate. c. Both of the above are true.Which lab technique would you use for each of the following: a.Break Open Cell Membrane b. Remove Cell Debris c.Amplify Region of Interest d. Analyze DNA for SNP VariationMatch the outcomes on the left with the laboratory steps on the right. ___ Harvest the cells A. Add salt ___ Dissolve cell membranes B. Add protease, incubate at 50°C ___ Precipitate the DNA C. Mix in a detergent solution ___ Break down proteins D. Layer cold alcohol over cell extract ___ Make DNA less soluble in water E. Gently chew the insides of your mouth, then rinse vigorously with water
- Which of the following correctly describes a possible scenario during a run of gel electrophoresis with DNA samples?In the isolation of DNA, which of the following is the function of chilled ethanol? a. chelate the Mg2+ ions that are essential for the overall integrity of the cell membrane b. break down cell membrane thus releasing intracellular structure c. precipitate the nucleoproteins through salting-out d. retard the nuclease activity then precipitate the DNACellular DNA replication uses the enzymes for the process while PCR uses thermal denaturation that helps seperate DNA strands. I need a picture that shows the difference between them two using the statement above.
- The percentage of the agarose gel is important because a) it influences the rate of migration of the fragments b) it may cause some DNA molecules to replicate c) some DNA nucleotides may degraded due to chemical reactions with the gel d) some DNA molecules may sink to the bottom of the well and not migrate What is the purpose of a buffer used in electrophoresis? a) Increase/decrease the current b) Conduct the current and protect the samples c) Disrupt the bands d) Stop the band migration Which of the following is an additional use of the gel electrophoresis technique? a) To express DNA b) To separate RNA c) To amplify RNA d) To amplify genesThe rate at which large DNA fragments move through the electrophoretic gel is ________ the rate at which small DNA fragments move through the same apparatus. a) Less than b) Greater than c) The same as d) None of the above After TRIZOL extraction and centrifugation, your tube looks like this. Which fraction should you collect if you want the RNA? a) “Cloudy”-looking interphase b) The aqueous phase c) The organic phase d) Everything except the organic phase How many different bases are associated with RNA? a) 5 b) 4 c) 3 d) 6What is the use of Liquid soap in DNA extraction?* A. binds cell membrane and nuclei B. dissolves DNA C. binds phosphate groups and salt D. none of the given options
- Which of the following is true of the gel electrophoresis shown? Note: Only one can be true... I'm between c or d. Previous answer confused me more. A) the power source has not been turned on yet. B) the three wells contained the same DNA molecules. C) well #1 had fewer molecules of DNA than well #2 or #3 D) well #3 had more different sized molecules of DNA than well #1 or #2Please answer the following using the experiment data below. Provide the formulas or methods used for calculations of each. Number of colonies on LB/amp/ara plate = Micrograms of DNA spread on the plates = Transformation efficiency = DNA plasmid concentration: 0.08 μg/μl 250 μl CaCl transformation solution 10 μl pGLO plasmid solution 250 μl LB broth 100 μl cells spread on agar 227 colonies of transformantsWhich DNA fragment will be closest to the top (negative pole) of anelectrophoretic gel?a. 450 bp b. 3,560 bp c. 5 kb d. 1,500 bp
