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- An unknown sample was titrated with CH3COOH. After making the titration curve, no steep change was observed. This means that the unknown sample must be a _____.Explain how gel filtration chromatography works. What type of gel will you used when the protein size is 2500 Da? Explain.If you mix two unknown samples and repeat the Lowry assay, is the absorbance equivalent to the sum of the two individual unknown samples that is used.
- How do you prepare 50 ml of 20 mg/ml proteinase K from 50 mg/ml stock solutionA DNA sample contains 21% adenine. What is the complete percentage base composition?What will happen if the protein that you are assaying using zymography is not fixed properly using Trichloroacetic acid, and which step of the zymography is crucial for obtaining clear white bands on a bluebackground?
- If I had a standard with 2.0 mL of Bradford reagent then added 50μL of 250 μL/mL ; what would be the concentration of original protein?In which reagent is extracted DNA suspended to put it in solution? A. Sodium citrate saline buffer B. Sodium dodecyl sulfate (SDS) C. Cold absolute alcohol D. HCl E. H2SO4How will you know when the endpoint of the titration is reached? Why is it necessary to mix the solution after you add base?. Single line text.
- Look for 5 color reaction/test that can be used to identify amino acids. Indicate also the color for positive results and what causes this results .When you graph your protein assay data in Excel, should you include the absorbance of your unknown BSA sample? Explain why or why notWhy is protein testing in human samples important?