Determine the effect (increase, decrease, no effect or cannot be determined) of the stated condition on the parameter indicated in the parentheses [Select] NaCl was not added during the extraction process (% yield of isolated DNA) Extracted DNA fibers were suspended in buffer of pH = 3.0 (% intact DNA) [Select] SDS was not added during extraction of DNA (%purity of isolated DNA) [Select] Incubation time of solution with ethanol was prolonged (% yield of isolated DNA) [Select] After ethanol precipitation, water was added instead of 70% ethanol (%purity of isolated DNA) [Select] After ethanol precipitation, water was added instead of 70% ethanol (%yield of isolated [Select] DNA)

Determine the effect (increase, decrease, no effect or cannot be determined) of the stated condition on the parameter indicated in the parentheses [Select] NaCl was not added during the extraction process (% yield of isolated DNA) Extracted DNA fibers were suspended in buffer of pH = 3.0 (% intact DNA) [Select] SDS was not added during extraction of DNA (%purity of isolated DNA) [Select] Incubation time of solution with ethanol was prolonged (% yield of isolated DNA) [Select] After ethanol precipitation, water was added instead of 70% ethanol (%purity of isolated DNA) [Select] After ethanol precipitation, water was added instead of 70% ethanol (%yield of isolated [Select] DNA)

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 18P: Functional Consequences of Y-Family DNA Polymerase Structure The eukaryotic translesion DNA...

See similar textbooks

Similar questions

P2. Calculate the frictional coefficient of a molecule of DNA of 20 base pairs in water at 20C; assume that the hydrodynamic behavior of the DNA itself can be approximated to be rod-like; the viscosity of the solvent is 0.01 g cm-1 s -1
give the significance/role/effect of the reagent/condition in the isolation or analysis of a biomolecule. higher absorbance at 260nm of melted DNA than the native DNA
Estimate the viscosith of 1.0 vol % agarose gel solution if it took 58 minutes for 1500 base pairs long DNA molecule to migrate 11cm during gel electrophoresis under 110V. Assume appropriate numerical values for the missing parameters such as the charge and diameter of DNA molecules.
Both DNA polymerase (any DNA polymerase) and ligase catalyze the formation of a bond between nucleotides, but these two enzymes do NOT catalyze the same reaction. Briefly describe the differencesbetween the reaction catalyzed by the polymerase activity of DNA polymerase and the one catalyzed by ligase.
INSTRUCTION: = IF BOTH STATEMENT ARE TRUE = IF FIRST STATEMENT IS TRUE WHILE SECOND STATEMENT IS FALSE = IF FIRST STATEMENT IS FALSE WHILE SECOND STATEMENT IS TRUE = IF BOTH STATEMENTS ARE FALSE STAMENT 1: If 3’ ATTG 5’ is transcribed, the complementary strands is 5’ UAAC 3’ STAMENT 2: Guanine and Cytosine are purine bases ANSWER: STAMENT 1: The general term for the enzyme that connects nucleotide triphosphates in DNA is DNA transferase STAMENT 2: The enzyme that unwinds the double stranded DNA is topoisomerase ANSWER: STAMENT 1: The name of the compound formed when uracil is bonded to ribose is uradine STAMENT 2: The piece of nucleic acid that is complementary to the DNA template and serves as a starting point of replication is called RNA promoter ANSWER:
Number of Okazaki Fragments in E. coli and Human DNA Replication Approximately how many Okazaki fragments are synthesized in the course of replicating an E. coli chromosome? How many in replicating an â€œaverageâ€� human chromosome?
Functional Consequences of Y-Family DNA Polymerase Structure The eukaryotic translesion DNA polymerases fall into the Y family of DNA polymerases. Structural studies reveal that their fingers and thumb domains are small and stubby (see Figure 28.10). In addition, Y-family polymerase active sites are more open and less constrained where base pairing leads to selection of a dNTP substrate for the polymerase reaction. Discuss the relevance of these structural differences. Would you expect Y-family polymerases to have 3-exonuclease activity? Explain your answer.
REFLECT AND APPLY Define processivity, and indicate the importance of this concept in DNA replication.
Compare the DNA binding modes of minor groove binding, intercalating, and crosslinking agents and explain how these interactions give rise to specific pharmaceutical applications. Use the molecular structures of at least one DNA PLEASE DISCUSS THIS IN DETAIL! and correct answers only ! will give good rating! thank you.
True or False? 454 platform sequencing involves attaching single -stranded DNA fragments to beads, which are embedded within a 96-well plate . The plate is exposed to separate washes of single nucleotides , which are used by DNA polymerase to synthesize complementary DNA. Each time a nucleotide is added, a pyrophosphate is released.
Please answer You have received a dehydrated sample of DNA primer at a concentration of 19.9 microM. what volume (in microlitres) of buffer would you add to achieve a solution of 100nM of this primer? Show workings.
Mutations in the dnaA gene of E. coli are lethal and canonly be studied following the isolation of conditional,temperature-sensitive mutations. Such mutant strains grownicely and replicate their DNA at the permissive temperatureof 18°C, but they do not grow or replicate their DNA at therestrictive temperature of 37°C. Two observations were usefulin determiningthe function of the DnaA protein product.First, in vitro studies using DNA templates that haveunwound do not require the DnaA protein. Second, if intactcells are grown at 18°C and are then shifted to 37°C, DNAsynthesis continues at this temperature until one roundof replication is completed and then stops. What do theseobservationssuggest about the role of the dnaA gene product?