A HEPTAPEPTIDE that punctures the bacterial cell wall has just been recently isolated from the venom gland of a frog. Amino acid analysis of the peptide reveals the following information below: (NOTE: when the sequence is not known, a comma separates the amino acids) Hydrazine Acid Hydrolysis (6 N HCI) at 110 °C Heptapeptide (2) Arg, Pro, Phe, Tyr, Ala, Lys 2,4-dinitrofluorobenzene DNP-Phe Lys and modified free Peptide 1 (Ala, Lys) amino acids Cleavage with Trypsin, then chromatography Peptide 2 (Pro, Arg, Tyr) Peptide 3 (Phe, Arg) Phe Cleavage with Chymotrypsin, then chromatography Peptide 4 (Arg, Tyr) Peptide 5 (Lys, Pro, Ala, Arg)

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A HEPTAPEPTIDE that punctures the bacterial cell wall has just been recently isolated from the
venom gland of a frog. Amino acid analysis of the peptide reveals the following information below:
(NOTE: when the sequence is not known, a comma separates the amino acids)
Hydrazine
Acid Hydrolysis (6 N HCI) at 110 °C
(2) Arg, Pro, Phe, Tyr, Ala, Lys
Heptapeptide
2,4-dinitrofluorobenzene
DNP-Phe
Lys and
modified free
Peptide 1
(Ala, Lys)
amino acids
Cleavage with Trypsin, then chromatography
Peptide 2
(Pro, Arg, Tyr)
Peptide 3
(Phe, Arg)
Phe
Cleavage with Chymotrypsin, then chromatography
Peptide 4
(Arg, Tyr)
Peptide 5
(Lys, Pro, Ala, Arg)
Transcribed Image Text:A HEPTAPEPTIDE that punctures the bacterial cell wall has just been recently isolated from the venom gland of a frog. Amino acid analysis of the peptide reveals the following information below: (NOTE: when the sequence is not known, a comma separates the amino acids) Hydrazine Acid Hydrolysis (6 N HCI) at 110 °C (2) Arg, Pro, Phe, Tyr, Ala, Lys Heptapeptide 2,4-dinitrofluorobenzene DNP-Phe Lys and modified free Peptide 1 (Ala, Lys) amino acids Cleavage with Trypsin, then chromatography Peptide 2 (Pro, Arg, Tyr) Peptide 3 (Phe, Arg) Phe Cleavage with Chymotrypsin, then chromatography Peptide 4 (Arg, Tyr) Peptide 5 (Lys, Pro, Ala, Arg)
When answering the questions below, please use the ONE-LETTER CODE for the amino acid, with NO
spaces and symbols between each letter.
1. What is the C-terminal residue?
2. What is the N-terminal residue?
3. What is the sequence of Peptide 3?
4. What is the sequence of Peptide 5?
5. What is the overall amino acid sequence?
Transcribed Image Text:When answering the questions below, please use the ONE-LETTER CODE for the amino acid, with NO spaces and symbols between each letter. 1. What is the C-terminal residue? 2. What is the N-terminal residue? 3. What is the sequence of Peptide 3? 4. What is the sequence of Peptide 5? 5. What is the overall amino acid sequence?
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