A mutated form of gene x has been isolated (X1). Its transcript abundance displays constitutive expression. Given this data, what is the nature of the X1 mutation? A. Mutation in 5' UTR that alters mRNA interaction with ribosomes B. Mutation in the protein-coding region C. Mutation in the promoter region D. Mutation in 3'UTR region
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- Which of the following statements best describes the promoter of a protein-coding gene? A. The promoter is a protein that recruits RNA polymerase. B. The promoter is part of the RNA molecule itself. C. The promoter is a site found on RNA polymerase. D. The promoter contains the AUG start codon. E. The promoter is a nontranscribed region of a gene.If a mutation occurred in a control element sequence associated with a particular gene, which of the following would be a likely consequence of this? A. This mutation is not likely to affect the expression of the associated gene, because RNA polymerase does not bind to control elements. B. This will likely affect the expression of the associated gene because it will loosen the coiling of chromatin at this site. C. The expression of the associated gene will be affected because this change will result in a nonsense mutation. D. This mutation is not likely to affect the expression of the associated gene, since control elements are noncoding sequences. E. The associated gene's expression would likely be affected, since this mutation would change the ability of at least one transcription factor to bind.a. How can a single eukaryotic gene give rise to several different types of mRNA molecules?b. Excluding the possible rare polycistronic message,how can a single mRNA molecule in a eukaryoticcell produce proteins with different activities?
- For each statement about gene expression mechanisms, choose the correct end to the sentence. For each gene, the template strand for transcription is determined by…. The direction of translation is determined by…… The tissue-specificity of protein production is determined by…. choices: a. location of the start codon b. location of the promoter c. direction of polymerization by RNA polymerase d. none of these e. direction of movement of ribosomes f. overall orientation of the chromosomeThe following is a portion of an mRNA sequence: 3’ –AUCGUCAUGCAGA-5’ a)During transcription, was the adenine at the left-hand side of the sequence the first or the last nucleotide used to build the portion of the mRNA shown? Explain how you know. b)Write out the sequence and polarity of the DNA duplex that encodes this mRNA segment. Label the template and coding DNA strands. c)Identify the direction in which the promoter region for this gene will be located.The “domain-swapping” experiment that grafts the Gal4 DNA-binding domain to the LexA activation domain generates a chimeric protein that will: A) bind to the Gal4 site. B) bindto the LexA site. C) activate transcription of the LexA gene. D) activate transcription of alleukaryotic genes. E) All of the answer options are correct.
- Which of the following mutations could be appropriately describedas a position effect?A. A point mutation at the –10 position in the promoter regionprevents transcription. B. A translocation places the coding sequence for a muscle-specificgene next to an enhancer that is turned on in nerve cells.C. An inversion flips a gene from the long arm of chromosome 17(which is euchromatic) to the short arm (which isheterochromatic).Consider the following mRNA base sequence 5' CUG-CAC 3' (a) What dipeptide is coded for by this mRNA? (b) What dipeptide is formed if a mutation converts CUG to CUU? (c) What dipeptide is formed if a mutation converts CAC to CGC? (d) What dipeptide is formed if a mutation converts CUG to CUU and CAC to CGC?Let's say there is an eukaryotic gene that has five exons and 4 introns. If the GU sequence at the 5' splice site of the third intron is deleted, describe how this is going to affect the expression of this gene. draw the mature mRNA too.
- Which of the following characteristics is typical of a eukaryotic gene that can be transcribed? a. The core promoter is wrapped around a nucleosome. b. The core promoter is found in a nucleosome-free region. c. The terminator is wrapped around a nucleosome. d. None of the above characteristics is typical of such a gene.a mutation in the gene encoding Dicer would most likely result in: A) a failure to break the H bonds holding the 2 strands of the siRNA together B) the inability to form H bonds betwen the siRNA and the complementary mRNA that it targets. C) the inability of the target mRNA to be cleaved upon H bonding with the siRNA D) a failure to produce siRNAs responsible for knocking down gene expression E) the failure to produce a dsRNA specific to your gene of interesta) what is a promoter and give the element and their functions of E.coli promoter b) what are eukaryotic transcription factor and list the class 2 general transcription factors and state their functions