a) Predict whether fumarate or maleate will have greater electrophoretic mobility. Cis-smaller than trans CO2 CO2 fumarate maleate b) Electrophoresis is run with the injection end positive and the detection end negative with a buffer with pH = 8.5. The charge on both ions is -2 at pH = 8.5. The electroosmotic flow is greater than the electrophoretic flow. Predict the order of elution of these two ions. At pH = 4.0, both anions have a charge of -1. At this pH, the injection end is negative, and the detection end is positive. Why is the polarity switched at lower pH?

a) Predict whether fumarate or maleate will have greater electrophoretic mobility. Cis-smaller than trans CO2 CO2 fumarate maleate b) Electrophoresis is run with the injection end positive and the detection end negative with a buffer with pH = 8.5. The charge on both ions is -2 at pH = 8.5. The electroosmotic flow is greater than the electrophoretic flow. Predict the order of elution of these two ions. At pH = 4.0, both anions have a charge of -1. At this pH, the injection end is negative, and the detection end is positive. Why is the polarity switched at lower pH?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

2. A sample of material contains for its active components NaOH, NazCO3, NaHCO3, or compatible mixtures of these. Two samples, each weighing 1.000 g, are dissolved in H20. To one sample phenolphthalein is added and the solution is titrated cold with 1.038 N acid, of which 17.96 mL is required. The other sample is titrated cold, with methyl orange as indicator, and 21.17 mL of the same acid is required. What alkalis are present, and what is the percentage of each in the original sample?
For the determination of Pb in blood, a 5.00 mL sample was treated with trichloroacetic acid to precipitate the proteins. After centrifugation, the resulting solution was brought to pH 3 and extracted with two 5 mL portions of methyl isobutyl ketone containing the lead complexing agent APCD (ammonium 1-pyrrolidine carbodithiolate). The extract was drawn directly into an air-acetylene flame which produced an absorbance of 0.444 at 283.3 nm. Two 5.00 mL aliquots of solutions containing 0.250 and 0.450 ppm of Pb were subjected to the same sample treatment and the absorbance that occurred was 0.396 and 0.599 respectively. Calculate the concentration of Pb in µg/dL in the blood.
In the preparation of a standard stock solution for protein analysis, 50 mg of bovine serum albumin (BSA) was dissolved in deionized distilled water to a final volume of 10 mL. What is the weight of BSA in 0.60 mL stock solution?
The isoelectric point of tyrosine is 5.63. Toward which electrode does tyrosine migrate on paper electrophoresis at pH 7.0?
1.What is the significance when KD is either a small or large value? 2. Methyl alcohol and ethyl alcohol are poor extraction solvents. Why?3. What is the major disadvantage of using ether as an extracting solvent?
At a pH of 10 what is the fraction of EDTA that is in the form Y4-? I got 0.30 but I believe that is wrong.
A 5.00 mL sample of filtered orange juice was treated with 50.00 mL of 0.01019 M I3– to oxidize ascorbic acid to dehydroascorbic acid. After the oxidation was complete, excess I3– was back- titrated with 13.96 mL of 0.07125 M Na2S2O3 to starch indicator endpoint. What is the concentration of ascorbic acid (MM = 176.12) in the orange juice? Report the concentration ascorbic acid in mg/100mL. C6H8O6 + I3– → C6H6O6 + 3I- + 2H+ I3– + 2S2O32- → S4O62- + 3I2 42.89 % 40.85 % 27.34 % 82.51 %
1) a) Predict whether fumarate or maleate will have greater electrophoretic mobility. b) Electrophoresis is run with the injection end positive and the detection end negative with a buffer with pH = 8.5. The charge on both ions is -2 at pH = 8.5. The electroosmotic flow is greater than the electrophoretic flow. Predict the order of elution of these two ions. c) At pH = 4.0, both anions have a charge of -1. At this pH, the injection end is negative and the detection end is positive. Why is the polarity switched at lower pH? Predict the order of elution of the two ions.
0.641 g of a semi-synthetic alkaloid was dissolved in 25 ml of 1% w/v acetic acid and wasanalysed directly by HPLC. The solution was found to contain 1.42 mg/100 ml of an impurity.What is the level of impurity in % w/w and ppm? Calculate the pH of a buffer system made by dissolving 1.2 g of acetic acid and 0.82 g ofsodium acetate in 500 ml of distilled water (pKa of acetic acid = 4.7)
Using the reagents, prepare 100mL solution with a concentration of 25.0 mM Tris buffer at a final pH of 7.70. •Solid Tris, FW = 121.1• Solid Tris-C1, the conjugate acid of Tris. It has a pKa of 8.30 (at 25° C) and a FW of 157.6. •deionized water
6. The quantitative determination of pyridoxine hydrochloride can be carried out by methods: A. Complexometry B. Argentometry C. Alkalimetry D. Acid-base titration in a protogenic solvent medium E. Acid-base titration in a profile solvent medium
At what pH would dissolved sulfate be equally distributed between HSO4 and SO24- ?