A solution containing 2g/liter of a light-absorbance substance in 1 cm cuvette transmits 75% of incident light of a certain wavelength. Calculate the transmission of a solution containing 4g / liter
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- For dispersion of light through a prism which colour has maximum deviation?In absorption spectrophotometry: a. Absorbance is directly proportional to transmittance b. Percent transmittance is directly proportional to concentration c. Percent transmittance is directly proportional to the light path length d. Absorbance is directly proportional to concentrationWhat color does an object appear if it absorbs light at both 420-500 nm and at 620-700 nm? a. Red b. Black c. Purple d. Green e. Deep blue
- Suppose you conduct direct visible spectrophotometry with red fruit punch as your solution. Which wavelengths of light would you expect to be strongly absorbed? Which wavelengths are not strongly absorbed? Explain.Assume that you have an “X” solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. Prepare a standard curve by using the absorbance of known concentrations (Hint: you can learn how to prepare a standard curve by using Excell from youtube). Calculate the concentration of unknown.Name the colors in a color spectrum of visible light from low to high wavelength.
- A typical incandescent lightbulb emits approximately 3 x 1018 visible-light photons per second. Your eye, when it is fully dark adapted, can barely see the light from an incandescent lightbulb 10 km away. How many photons per second are incident at the image point on your retina? The diameter of a dark-adapted pupil is 6 mm.Which letter on the figure below represents the light intensity control?In an experiment of analysis of bovine serum albumin the spectrophotometer will be set to measure the light absorbed at 595 nm only, rather than a variety of different wavelengths of light. If you have measured the light absorbed at 580 nm for all of samples instead, how does it affect your results and consequently final result? Explain your answers in details.
- Match the following items: [4] 1. 540 nm A. Wavescan 2. Quantity of light of a specific wavelength absorbed by a sample B. Standard series 3. Reference samples with a concentration range C. Absorbance 4. Absorbance values at all wavelengths D. PurpleDescribe the absorption of light in your own words.If we used the 365nm setting on the UV light box would this have caused more or less UV light damage
