Match the following items: [4]1. 540 nmA. Wavescan2. Quantity of light of a specific wavelength absorbed by a sampleB. Standard series3. Reference samples with a concentration rangeC. Absorbance4. Absorbance values at all wavelengthsD. Purple

The absorbance of a solution of a biological molecule is determined by Lambert-Beer law. The law…

Answered: Match the following items: [4] 1.…

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter15: Instrumentation In Medical Imaging

Section: Chapter Questions

Problem 7Q

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Discuss the types of interferences in atomic absorption spectrophotometry.
Some spectrophotometers use wavelengths of light in the ultraviolet range. What are wavelengths of light that are in the ultraviolet range?
Assume that you have an “X” solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. Prepare a standard curve by using the absorbance of known concentrations (Hint: you can learn how to prepare a standard curve by using Excell from youtube). Calculate the concentration of unknown.
Suppose you were in a research lab in Romania and the lab is equipped with several spectrophotometers (UV and VIS). You need to determine the concentration of a protein solution and although you know several techniques you must decide which spectrophotometer to use. Which spectrophotometer are you choosing and which wavelength will you use?
FOV measured at 100x total magnification is 400 micrometers. What is the FOV at 40X total magnification? 2. If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?
a. The scale of a spectrophotometer extends from 1 to 100% T, what are the values of these two extremes in absorbance? b. Convert the 98%T to absorbance
With regard to the spectrophotometer, which of the following is true for regarding the term absorbance? I. The term refers to the amount of light that is prevented from passing through a solution. II. As solute concentration increases the absorbance reading also increases. III. Absorbance is the opposite of transmittance.
Suppose you conduct direct visible spectrophotometry with red fruit punch as your solution. Which wavelengths of light would you expect to be strongly absorbed? Which wavelengths are not strongly absorbed? Explain.
If a researcher forgot to wipe the outside a sample cuvette to make it free of drops, fingerprints, and dust prior to reading it in the spectrophotometer, how might the absorbance value of the sample be affected? (Higher, Lower, No Change) Explain
A calibration curve for the absorbance of an unknown is shown below. What would be the concentration of a sample with absorbance of 0.45. Give the answer with the correct unit.
In absorption spectrophotometry: a. Absorbance is directly proportional to transmittance b. Percent transmittance is directly proportional to concentration c. Percent transmittance is directly proportional to the light path length d. Absorbance is directly proportional to concentration
In an experiment of analysis of bovine serum albumin the spectrophotometer will be set to measure the light absorbed at 595 nm only, rather than a variety of different wavelengths of light. If you have measured the light absorbed at 580 nm for all of samples instead, how does it affect your results and consequently final result? Explain your answers in details.

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1. 540 nm	A. Wavescan
2. Quantity of light of a specific wavelength absorbed by a sample	B. Standard series
3. Reference samples with a concentration range	C. Absorbance
4. Absorbance values at all wavelengths	D. Purple

Match the following items: [4] 1. 540 nm A. Wavescan 2. Quantity of light of a specific wavelength absorbed by a sample B. Standard series 3. Reference samples with a concentration range C. Absorbance 4. Absorbance values at all wavelengths D. Purple