A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the λmax of Ben, and 0.463 at the λmax of Jerry. STD CURVE BEN Λmax Ben Λmax Jerry STD CURVE JERRY Λmax Ben Λmax Jerry CONC (mM) ABS ABS CONC (mM) ABS ABS 100 0.074 0.025 100 0.037 0.074 200 0.148 0.057 200 0.081 0.148 400 0.284 0.103 400 0.164 0.284 800 0.607 0.218 800 0.287 0.607 1600 1.457 0.499 1600 0.678 1.457 Using the given data above, determine the following information: What is the λmax of Jerry? 800 nm 500 nm 700 nm 650 nm
A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties.
|
Absorbance |
||
|
Wavelength |
Compound Ben 500 mM |
Compound Jerry 500 mM |
|
400 nm |
0.137 |
0.136 |
|
450 nm |
0.312 |
0.113 |
|
500 nm |
0.154 |
0.078 |
|
550 nm |
0.076 |
0.079 |
|
600 nm |
0.227 |
0.148 |
|
650 nm |
0.230 |
0.230 |
|
700 nm |
0.151 |
0.357 |
|
750 nm |
0.157 |
0.246 |
|
800 nm |
0.154 |
0.154 |
A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the λmax of Ben, and 0.463 at the λmax of Jerry.
|
STD CURVE BEN |
Λmax Ben |
Λmax Jerry |
|
STD CURVE JERRY |
Λmax Ben |
Λmax Jerry |
|
CONC (mM) |
ABS |
ABS |
|
CONC (mM) |
ABS |
ABS |
|
100 |
0.074 |
0.025 |
|
100 |
0.037 |
0.074 |
|
200 |
0.148 |
0.057 |
|
200 |
0.081 |
0.148 |
|
400 |
0.284 |
0.103 |
|
400 |
0.164 |
0.284 |
|
800 |
0.607 |
0.218 |
|
800 |
0.287 |
0.607 |
|
1600 |
1.457 |
0.499 |
|
1600 |
0.678 |
1.457 |
Using the given data above, determine the following information:
What is the λmax of Jerry?
|
800 nm |
||
|
500 nm |
||
|
700 nm |
||
|
650 nm |
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