A suspected pathogen is observed from the blood of a diseased mouse. An investigation of the other mice in the area indicates that it is not present in healthy mice. With Koch's postulates in mind, what is the next step to determine whether or not this suspected pathogen is the cause of disease in the mouse? a) Grow the suspected pathogen in a pure culture Ob) Re-isolate the suspected pathogen and show that it is the same as the original pathogen c) Inoculate a healthy mouse with the suspected pathogen O d) Determine whether the organism in the pure culture is the same one as in the original sample

A suspected pathogen is observed from the blood of a diseased mouse. An investigation of the other mice in the area indicates that it is not present in healthy mice. With Koch's postulates in mind, what is the next step to determine whether or not this suspected pathogen is the cause of disease in the mouse? a) Grow the suspected pathogen in a pure culture Ob) Re-isolate the suspected pathogen and show that it is the same as the original pathogen c) Inoculate a healthy mouse with the suspected pathogen O d) Determine whether the organism in the pure culture is the same one as in the original sample

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter9: Microbial Disease Transmission

Section: Chapter Questions

Problem 1UTM: As an illustration, a patient undergoing a laparoscopic-assisted vaginal hysterectomy (LAVH)...

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Why is aseptic urine collection important when cultures are ordered? If you counted 20 colonies from a 0.01-ml inoculum of a 1:10 dilution of urine, how many organisms per milliliter of specimen would you report? Is this number significant? What can you learn from visual inspection of a urine specimen? How would you relate these to the microorganisms present in the sample? How are UTIs acquired/transmitted? Explain why E. coli is frequently implicated in cystitis in females.
Before development of a vaccine against this microbe, thedisease it caused accounted for two-thirds of bacterial meningi-tis cases during the first year of life but is still the number oneleading cause of mental retardation in patients who survive seri-ous disease due to permanent central nervous system disorders.What is the microorganism?(a) Haemophilus influenzae type B(b) Haemophilus influenzae type A(c) Neisseria meningitidis(d) Streptococcus pneumoniae(e) Listeria monocytogenes
Explain the role of pylori in the pathogenesis of peptic ulcers. Which populations are most susceptible to peptic ulcers? Is H. pylori normal flora? If so, what causes the species to become pathogenic? Construct at table to compare and contrast the 5 steps of microbial pathogenesis. How does extracellular growth differ from intracellular growth? Diagram the 5 step pathogenesis cycle for coli O157:H7, an extracellular, intestinal pathogen acquired by consuming contaminated food/water. Be sure to include the role of exoenzymes and the Shiga exotoxin in your diagram. (2 points) Explain the pathogenesis of Listeria monocytogenes. Be sure to include temperature regulation, intracellular growth, and at risk groups in your discussion.
Functionally, normal microbiota is beneficial to it's host organism and on the other hand, it may cause harm, How does this normal microbiota cause harm? Explain briefly but acurately
What is the definition of the term Resistant? How do you detect that your microorganism is resistant to a certain antibiotic? What do you see on the Muller Hinton Agar plate?
The patient, who presented with complaints such as sore throat, fever, weakness, and swollen tonsils, was examined by an ear, nose and throat doctor. Having diagnosed the patient with Streptococcus pyogenes pharyngitis, the doctor requested a throat culture from the patient to confirm the accuracy of the diagnosis. a. In which environment does the Laborant develop Streptococcus pyogenes in the throat culture taken from the patient with a swab? Explain by explaining. b. Laborant is Strep in mixed culture, which is formed as a result of throat culture that he developed (cultivated). Using which methods to analyze and determine pyogenes phenotypically? c. Phenotypically detected Strep. The laboratory technician, who differentiates the pyogenes from other bacterial species in the environment, develops the bacteria in which specific environment using which method? Explain by explaining.
As an illustration, a patient undergoing a laparoscopic-assisted vaginal hysterectomy (LAVH) procedure under general anesthesia might be happier not to know the numbers of portals of entry for potential transmission of pathogenic microbes to which she will be subjected. The anesthesia provider would be accessing the patients airway and vascular system by an IV line. Which portals of entry will the surgeon be accessing?
Below are a list of virulence factors/ strategies paired with an example of an organism that utilizes them. How do each of the following strategies contribute to the virulence of the pathogen? Strategy - Causes the host to produce more receptors (Organism - Rhinovirus) Strategy - Produces gas as a product of fermentation (Organism - Clostridium perfringens) Strategy - Produces a capsule (organism - Klebsiella pneumonia) Strategy - Ability to move between adjacent cells (organism - Cytomegalovirus) Strategy - Ability to use pilus as a motility structure (organism - Pseudomonas aerogenosa)
Different strains of Streptococcus pyogenes have different virulence factors, giving these microbes much versatility. Virulence factors include which of the following? Choose one or more: A.A capsule that, when thick, will help the organism avoid phagocytosis by macrophages B.A cell wall containing lipoteichoic acid, thought to facilitate adherence to pharyngeal epithelial cells C.Enzymes that lyse blood cells (streptolysins) D.The production of several endotoxins called streptococcal pyogenic endotoxins (SPEs)
What makes bacterial diseases such as Clostridium botulinum, Clostridium perfringens & Clostridium tetani very effective to cause infection in a human host?
An outbreak of Methicillin-resistant Staphylococcus aureus (MRSA) in the surgical ward at the hospital forces the hospital administrator to cancel and postpone all non-essential surgeries until the source of infection has been located and for measure put into place. Bacterial samples obtained from swab of wad staff equipment and surfaces arr brought to the lab for analysis. 1. What testing method will you employ to determine the level of exposure? 2 How would a positive test present? 3. How would a negative test present? 4. What recommendations would you make in your report?
What are the common pathogens isolated from stool samples? What is the difference between a coliform bacterium and a noncoliform enteric bacterium? What diagnostic test differentiates Proteus and Providencia species from other Enterobacteriaceae? How would you differentiate between serotypes of E. coli? Are the gram-negative enteric bacilli fastidious organisms? Would they survive well outside of the body? If so, what significance would this have in their transmission? Why is serotyping particularly important in Salmonella infections and typhoid fever?