(a) What are the Vmax and Km (including units) for Tigerzyme? b) What are the Vmax and Km (including units) for Tigerzyme in the presenceof the inhibitor Tar Heel? (c) What are the Vmax and Km (including units) for Tigerzyme in the presenceof the inhibitor Blue Devil?
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
(a) What are the Vmax and Km (including units) for Tigerzyme?
b) What are the Vmax and Km (including units) for Tigerzyme in the presenceof the inhibitor Tar Heel?
(c) What are the Vmax and Km (including units) for Tigerzyme in the presenceof the inhibitor Blue Devil?
![14. You have decided to join my laboratory for a two semester BIOCH 491 project. Your
project is to kinetically characterize the enzyme Tigerzyme. Through careful analysis,
have demonstrated that Tigerzyme follows Michaelis-Menten kinetics. We have
previously shown that Tigerzyme has two inhibitors Tar Heel and Blue Devil. You obtained
the following kinetic data for Tigerzyme in the absence of inhibitor, in the presence of
the inhibitor Tar Heel, and in the presence of the inhibitor Blue Devil. Assume [ET] is the
same for each experiment. A graph sheet is provided on the next page.
you
No inhibitor added
Tar Heel added
Blue Devil added
1/[S] (mM)
1/V (mmol/min)
1/V (mmol/min)
1/V (mmol/min)
1
0.4
0.85
1.3
0.5
0.25
0.48
0.78
0.2
0.16
0.25
0.5
0.1
0.13
0.18
0.4
0.05
0.11
0.14
0.35](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F279d9c1a-8686-4970-a64d-6e4fa13a7ad9%2F06ab4014-668f-4089-9425-f10f90e9000f%2F8apk569_processed.png&w=3840&q=75)
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