(a) Why the plasmid (containing the foreign DNA), together with the competent bacterial cells are heated at 42°C? b) What would be the next stage after heat shock? Discuss the process involved. c) How would you analyze the efficiency of the competent cells used during transformation?

(a) Why the plasmid (containing the foreign DNA), together with the competent bacterial cells are heated at 42°C? b) What would be the next stage after heat shock? Discuss the process involved. c) How would you analyze the efficiency of the competent cells used during transformation?

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 6SQ

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Concept explainers

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.

Question

(a) Why the plasmid (containing the foreign DNA), together with the competent
bacterial cells are heated at 42°C?

b) What would be the next stage after heat shock? Discuss the process involved.

c) How would you analyze the efficiency of the competent cells used during
transformation?

The figure below shows the heat-shock method, which is a process involved during DNA
transformation into Escherichia coli (E. coli).
42 C 5$42 °C heat-shock for 1 min
lee bath for 5 min
Classic heat-shock
lce bath for 3 min

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