Q: 1. (a) Restriction sites are usually ______. Recombinant DNA Technology Restriction…
A: Given, 1. (a) Restriction sites are usually ______. Recombinant DNA Technology…
Q: Define the following terms:a. hypochromic effectb. DNA denaturationc. restriction endonucleasesd.…
A: Recombinant DNA is made by combining two or more DNA from other sources. The process depends on the…
Q: Which of the following objects would you use microinjection in order to transfer DNA into? a. Egg…
A: Microinjection is the process of transferring DNA materials into a living cell. It can be done by…
Q: Which statement is NOT the function of restriction enzyme?
A: Answer: It ligates the desired to gene to vector’s plasmid.
Q: How is bacterial DNA safe from digestion by Restriction Endonucleases? The plasmid of the…
A: Question- How is bacterial DNA safe from digestion by Restriction Endonucleases? A) The…
Q: When a 5-kb circular plasmid is digested with a restriction enzyme that has three recognition sites…
A: The restriction enzymes are present in bacteria and in other prokaryotes. The restriction enzymes…
Q: DEFINE THE FOLLOWING: 1) restriction enzyme 2) plasmid 3) recombinant DNA
A: Ans 1) Restriction enzymes are enzymes that are isolated from bacteria that can cleave…
Q: You have a DNA sequence of 1800 bp. To characterize it, you decide to make a restriction map.…
A: Restriction mapping is a process that helps to determine the location of the restriction site. The…
Q: Single and double digestion of plasmid pJC716 were performed using the restriction enzymes EcoRI and…
A: Plasmid is a double stranded circular dna molecule that is capable of replicating independently. It…
Q: Sequence of which of the following cannot be determined using the Maxam Gilbert method?a) Bacteriab)…
A: In the year 1973, Walter Gilbert and Allan Maxam developed DNA sequencing method by chemical…
Q: Why are the following reagents used? Neutralizing solution (Plasmid isolation) Isopropanol…
A: 1) Also, the neutralisation of the arrangement permits the renaturation of DNA. The enormous…
Q: Please answer the following using the experiment data below. Provide the formulas or methods used…
A: Transformation efficiency is defined as the number of colonies obtained per microgram of DNA plated.
Q: Chromosomal DNA fragments for molecular cloning are cut by _________, and inserted into molecular…
A: Answer - Option B - Restriction endonucleases; Ligase
Q: Which technique used to manipulate genetic material results in a significant increase in DNA or RNA…
A: In biotechnology laboratories different techniques are used to manipulate the genome of the…
Q: A plasmid DNA and a linear DNA (both of the same size) have one site for a restriction endonuclease.…
A: Because plasmid is a circular DNA molecule, this is the case. It becomes linear but not fragmented…
Q: Which of the following would allow for control of a gene's orientation when inserted into a plasmid…
A: The technique of molecular biology used to generate similar copies of a particular segment (gene) of…
Q: Select all the following statements that are correct regarding agarose gel electrophoresis and…
A: Agarose gel electrophoresis is a process used for separation of DNA fragments. Steps of agarose gel…
Q: A. Use this data to generate a circular map of the plasmid. Pst I: 10 kb Bam HI: 10 kb Sal I:…
A: Restriction endonucleases are enzymes that cut DNA at specific sites. Restriction endonucleases…
Q: Which of the following would you need to clone a human gene? Choose all that apply A restriction…
A: Gene cloning is the interaction wherein a suitable gene is found and replicated (cloned) out of all…
Q: Which of the following can be termed as a restriction modification system?a) Restriction…
A: Enzymes are the biological catalysts which are proteinaceous in nature. The function of an enzyme is…
Q: tarting material, how many restriction sites were there considering you observed three bands after…
A: Restriction enzyme or restriction endonucleases act on certain specific sites present in the double…
Q: See the restriction enzyme map below. The total DNA length is 1800 base pairs. If this DNA is cut…
A: An enzyme derived from bacteria that cuts DNA molecules at specified sequences is known as a…
Q: ligated into plasmid vectors and transformed into bacterial cells/colonies on agar plates. This…
A: Answer is (D) Gene Library A gene library is a collection of total genomic DNA from an organism in…
Q: Technique whereby inserting DNA into a clone is accomplished using two different restriction enzymes
A: Directional cloning is the technique which is accomplished by cleaving the plasmid vector with two…
Q: Compare and contrast the following terms: cDNA and gene Restriction fragment and gene DNA probe and…
A: Gene expression is a process in which the genetic instructions of genes are utilized to manage…
Q: If the F plasmid does not have the gene encoding Fino, this F plasmid would Select one: a. become…
A: Fertility factor or F factor or F plasmid is a sex factor that helps to transfer the genes between…
Q: Define single nucleotide polymorphism (SNP), restriction enzyme, cathode, anode, agarose, well, and…
A: All these are components are used in gel electrophoresis Gel electrophoresis is a technique in…
Q: Restriction maps illustrate the lengths of DNA fragments between restriction sites. Which of the…
A: Restriction mapping is a technique to map unknown segment of DNA by breakung it into small…
Q: In a transformation experiment, a sample of competent bacteria was mixed with a plasmid containing…
A: In a bacterial colony some bacteria are resistant to an antibiotic and some are susceptible.…
Q: DNA samples from four individuals were cleaved with the same MW restriction endonuclease. The DNA…
A: Any human biological specimen taken or preserved for the purpose of extracting and analysing DNA in…
Q: -gene--- 5'-GTCGACAAGCTTAAGCCTAAGCTTTAGCGAAGCTTGTCGAC-3' Recognition sites Sall 5'-GTCGAC-3'…
A: Sticky ends are short single-stranded overhanging segments of one DNA strand after the Double DNA is…
Q: The map of plasmid pUC19 is shown below. The restriction site coordinate is the position of the…
A: Introduction Plasmid Vectors: these are like the artificial vehicle to deliver our gene of insert…
Q: Restriction enzymes... Choose the answer below: cut DNA molecules at random sites. are…
A: DNA or deoxyribonucleic acid is a double-stranded molecule, strands of which are coiled around one…
Q: You are studying a new plasmid, and you digest the plasmid with three restriction enzymes: Eco RI…
A: The plasmid map can be created by checking the individual band patterns and band patterns with a mix…
Q: You are studying a new plasmid, and you digest the plasmid with three restriction enzymes: Eco RI…
A: Introduction: Gel electrophoresis: The technique of separating DNA fragments as per their size is…
Q: describe the two main parts of a plasmid DNA extraction and purification procedure including the…
A: Plasmid DNA extraction
Q: The transformation results below were obtained with 10 ul of intact plasmid DNA at nine…
A: Transformation Transformation is the process by which exogenous DNA is transferred into the host…
Q: Cuts the DNA into fragments Circular DNA molecule of bacteria Used to insert DNA of interest to…
A: Restriction enzyme - When this enzyme identifies its target sequence, it will form a double-stranded…
Q: Which restriction endonucleases would cleave a DNA molecule at the given sequences? The…
A: Restriction endonucleases are found in bacteria and they cut double-stranded DNA at the…
Q: Which of the following single-stranded DNA molecules may possibly be a restriction enzyme target cut…
A: Restriction enzymes are specific endonuclease that are responsible for cutting double standard DNA…
Q: Plasmids used in recombinant DNA technology typically: (MARK ALL THAT APPLY) Group of answer…
A: Plasmids are extrachromosomal DNA which replicate autonomously in side the cells. Plasmids are…
Q: The recognition site of some restriction enzymes are shown. Which will produce sticky ends? A.…
A: Restriction enzymes also known as restriction endonucleases are the enzymes which acts on the…
Q: The total size of the plasmid is 2686 bp. There is a PstI recognition site at position 439, HindIII…
A: Answer - d. 3 fragments: 447bp, 507bp and 1732bp
Q: In the following "gene library" cloning experiment Digested genomic DNA AmpR gene TCR gene TCR is…
A: Introduction The combining of DNA molecules from two distinct species is known as recombinant DNA…
Q: Which of the following is true about restriction endonucleases?a) Type I and II requires ATP to move…
A: Restriction endonucleases are the specific DNA sequences that cleaves the sugar-phosphate backbone…
Q: Which vectors (plasmid, phage λ, cosmid, bacterial artificial chromosome) can be used to clone a…
A: In molecular cloning, a vector is a molecule of DNA used as a vehicle to carry foreign genetic…
Q: Which of the following isoforms has faster rate of renaturation given the same concentration?…
A: The rate of renaturation of genomic DNA is dependent on the complexity of the genome.
Q: A segment of DNA is isolated and added to a 01 mixture of four deoxynucleotides, two specific…
A: The correct option is D.
Q: In order to insert foreign DNA into a plasmid for molecular cloning, the main components required…
A: Plasmids are small, double stranded, closed circular, symbiotic DNA molecules that occur naturally…
Define the following terms:
- Genome
- Bacteriophage
- λ DNA
- Plasmid
- Restriction Enzyme
- Standard Curve
- Plasmid Map
Step by step
Solved in 2 steps
- Define the following terms: Plasmid Restriction Enzyme Standard CurveDefine the following terms:a. hypochromic effectb. DNA denaturationc. restriction endonucleasesd. DNA hybridizatione. Southern blottingWhich bacterial enzyme removes the primers?a. Primaseb. DNA polymerase Ic. DNA polymerase IIId. Ligase
- Define the following terms:a. DNAb. RNAc. double helixd. genomee. transcriptionSequence of which of the following cannot be determined using the Maxam Gilbert method?a) Bacteriab) Plantsc) Bacteriophage T7d) PlasmidSelect the sequences that would be recognized by a restriction enzyme? (There can be more than one answer) A) 3'-TAGCTA-5' B) 5'CGATTC-3' C) 5'GAATTC- 3' D) 5'GAGCTC-3'
- Using one of the options below, finish the following statement so that it is correct. Plasmids are _________ a. extrachromosomal, circular molecules of RNA b. extrachromosomal, linear molecules of DNA c. None of these options are correct d. extrachromosomal, circular molecules of DNA e. extrachromosomal, linear molecules of RNA1. produces multiple identical copies of a gene [ Choose ] a. DNA ligase b. gene cloning c. gel electrophoresis d.restriction enzymeswrite in one or two sentence for each please. Define the following terms:a. biotechnology: ____________________________________________________________b.genetic engineering: ________________________________________________________c. sticky ends: ______________________________________________________________d. plasmid: ________________________________________________________________e. recombinant DNA: _________________________________________________________f. genetic marker: ___________________________________________________________g. transgenic organism: _______________________________________________________h. clone: __________________________________________________________________i. vector: ________________________________________________________________