a) Write an equation for the reaction catalyzed by fumarate reductase. b) NADH produced by the glyceraldehyde-3-phosphate dehydrogenase reaction is re-oxidized by reducing an organic intermediate. Rather than reduce pyruvate to lactate, anaerobic E. coli utilize fumarate reductase. However, under anaerobiosis, the activity of -ketoglutarate dehydrogenase is virtually nonexistent. Show how fumarate is formed, using a reaction beginning with PEP and including the necessary TCA cycle enzymes. c) What is the metabolic advantage to anaerobic E. coli in using the fumarate reductase pathway rather than lactate dehydrogenase to re-oxidize NADH?
Under anaerobic conditions, Escherichia coli synthesizes an NADH-dependent fumarate reductase rather than succinate
dehydrogenase, the flavoprotein that oxidizes succinate to fumarate.
a) Write an equation for the reaction catalyzed by fumarate reductase.
b) NADH produced by the glyceraldehyde-3-phosphate dehydrogenase reaction is re-oxidized by reducing an organic
intermediate. Rather than reduce pyruvate to lactate, anaerobic E. coli utilize fumarate reductase. However, under
anaerobiosis, the activity of -ketoglutarate dehydrogenase is virtually nonexistent. Show how fumarate is formed, using
a reaction beginning with PEP and including the necessary TCA cycle enzymes.
c) What is the
dehydrogenase to re-oxidize NADH?
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