a) write down the isolation protocols of salmonella Typhi and salmonella Typhimurium. (b) list 5 biochemical characteristics in which S.Typhimurium BIOCHEMICALLY differs from S.typhi.
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- In this lab exercise, were the results of the indole test necessary to differentiate between Salmonella and Shigella? Explain why or why not.Why is CFU more applicable to a culture of Streptococcus than to a culture of E. coli?Which coliform bacteria are the most difficult to distinguish from the Salmonella or Shigella pathogens? What is the primary characteristic used to differentiate them?
- Determine the optimal pH (or range of pH) for: Escherichia coli and Staphylococcus aureusWhy do Salmonella growing on XLT-4 agar is red colony with black center. Explain it in detail and concisely.Why is aseptic urine collection important when cultures are ordered? If you counted 20 colonies from a 0.01-ml inoculum of a 1:10 dilution of urine, how many organisms per milliliter of specimen would you report? Is this number significant? What can you learn from visual inspection of a urine specimen? How would you relate these to the microorganisms present in the sample? How are UTIs acquired/transmitted? Explain why E. coli is frequently implicated in cystitis in females.