The isolation of Salmonella Typhimurium and S.Typhi require different protocols: A) In what aspects do these protocols differ? B) why do these protocols differ? C) How will you be able to distinguish between these organisms on XLD and Hektoen Agar?
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The isolation of Salmonella Typhimurium and S.Typhi require different protocols:
A) In what aspects do these protocols differ?
B) why do these protocols differ?
C) How will you be able to distinguish between these organisms on XLD and Hektoen Agar?
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- How does bile in bile esculin medium contibute to the isolation and presumptive identification of Enterococcus?Why is it necessary to perform an enrichment procedure in isolating Staphylococcus aureus instead of directly plating it into a selective medium? What might happen if you omit potassium tellurite in using GCM?Why is aseptic urine collection important when cultures are ordered? If you counted 20 colonies from a 0.01-ml inoculum of a 1:10 dilution of urine, how many organisms per milliliter of specimen would you report? Is this number significant? What can you learn from visual inspection of a urine specimen? How would you relate these to the microorganisms present in the sample? How are UTIs acquired/transmitted? Explain why E. coli is frequently implicated in cystitis in females.
- What is the advantage of using Molecular data compared to plated culture or biochemical techniques in identifying bacterial species, especially for oral microbes ?McConkey agar red is considered to be both differential and selective for the isolation of members of the family Enterobacteriaceae. Explain whyWhat are the common pathogens isolated from stool samples? What is the difference between a coliform bacterium and a noncoliform enteric bacterium? What diagnostic test differentiates Proteus and Providencia species from other Enterobacteriaceae? How would you differentiate between serotypes of E. coli? Are the gram-negative enteric bacilli fastidious organisms? Would they survive well outside of the body? If so, what significance would this have in their transmission? Why is serotyping particularly important in Salmonella infections and typhoid fever?
- What type of reaction or lab test differentiates the presence of either staphylococcus bacteria or streptococcus bacteria in microbiology?After inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Choose ALL that apply) a - There is fungal growth in the original broth culture tube. b- There is too much growth on the agar slant. c- There are colonies of similar morphology on the slant. d - There are red, yellow, and white colonies on the slant. e - There is no growth on the slant.Why are aseptic techniques implemented during inoculation?
- A microbiologist used the concept of enrichment culture to isolate aerobic and anaerobic nitrogen-fixing bacteria, sulfate-reducing bacteria, and sulfur-oxidizing bacteria. What kind of selective media could he haveused for isolating each of these four classes of microbes?Why can EMB agar be used to detect the presence of E. coli in particular? Explain the principle of detection of the organism using EMB agarWhat are two possible reasons for choosing a bacteriostatic treatment over a bactericidal one? Name the factors that can compromise the effectiveness of a disinfecting agent