6. A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography. Whensubjected to gel electrophoresis in the presence of SDS the protein gives three bands with molecular mof 180, 160, and 60 kDa. When electrophoresis is carried out in the presence of SDS and dithiothreitol(DTT), three bands again form, this time with molecular masses of 160, 90, and 60 kDa.a. Approximately how many amino acids are present in a 400 kDa protein. (show work)b. How many grams would there be in a nanomole of this protein? (show work)C.What is the purpose of adding DTT?d. How many subunits does the protein have, and what are their molecular weights?

Gel electrophoresis Gel electrophoresis is a technique used for the separation of proteins based on…

a. Approximately how many amino acids are present in a 400 kDa protein. (show work) b. How many grams would there be in a nanomole of this protein? (show work) What is the purpose of adding DTT? d. How many subunits does the protein have, and what are their molecular weights?

a. Approximately how many amino acids are present in a 400 kDa protein. (show work) b. How many grams would there be in a nanomole of this protein? (show work) What is the purpose of adding DTT? d. How many subunits does the protein have, and what are their molecular weights?

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter14: Dna Structure And Replication

Section14.2: Dna Structure

Problem 2SB

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Question

6. A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When
subjected to gel electrophoresis in the presence of SDS the protein gives three bands with molecular m
of 180, 160, and 60 kDa. When electrophoresis is carried out in the presence of SDS and dithiothreitol
(DTT), three bands again form, this time with molecular masses of 160, 90, and 60 kDa.
a. Approximately how many amino acids are present in a 400 kDa protein. (show work)
b. How many grams would there be in a nanomole of this protein? (show work)
C.
What is the purpose of adding DTT?
d. How many subunits does the protein have, and what are their molecular weights?

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