1. What are the three general steps involved in isolation of proteins? Discuss or briefly describe the purpose of each step.
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1. What are the three general steps involved in isolation of proteins? Discuss or briefly describe the purpose of each step.
2. Suggest a simple procedure (or test) to verify if the final product is indeed casein.
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- 1. What is the name of the method you used to isolate protein from bacterial samples? 2. Give the protocol you use to successfully achieve our protein isolation. 3. In a polyacrylamide gel electrophoresis, the gel consists of specific gel components, name them.1. Suppose Biuret test is conducted to a solution of RNA. Will it give a positive result or not? Explain your answer.Explain how to prepare 2 ml of a solution with a concentration of 1 μg/5ml from a stock solution of a DNA sample with a concentration of 0.1 mg/ml.
- How could you improve upon your technique in order to get a better and more accurate standard curve graph? 2-Estimate (from your data) the [Protein] of your unknown based on comparing to the standards. Include the correct unit. This estimate should be an integer (no decimals).1. Why do we need to check the isolated DNA for its quantity and quality? 2. The purity of DNA sample is below 1.8 A260/A280 so, where did the proteincontamination come from? Note: Cite the in-text citation and referencesThe physician ordered platinum 100 mg/m2 iv to infuse over 6 hours once 4 weeks for a patient who has a bsa of 1.66m2. (a) how many mg of this antineoplastic drug should the patient receive? (b) if the dose of cisplatin were administered in 1,000 ml d5 1/2 ns, at how many ml/hr would the iv run?
- Cite the qualitative tests to characterize the products of RNA hydrolysis. Indicate the reagents involved in the process and indicate the positive result a. Bial's Test b. Murexide TestCite the qualitative tests to characterize the products of RNA hydrolysis. Indicate the reagents involved in the process and indicate the positive result: A. Bial's test B.Murexide TestWhen Griffith injected mice with a combination of live rough-strain and heat-killed smooth-strain pneumococci, he discovered that (a) the mice were unharmed (b) the dead mice contained living rough-strain bacteria (c) the dead mice contained living smooth-strain bacteria (d) DNA had been transferred from the smooth-strain bacteria to the mice (e) DNA had been transferred from the rough-strain bacteria to the smooth-strain bacteria
- ISOLATION AND CHARACTERIZATION OF NUCLEIC ACIDS (DNA) Explain the principle involved in the following chemical tests for the characterization of DNA a. Test for deoxyribose b. Test for phosphate c. Test for purines d. Test for pyrimidinesIn your forensic laboratory, you set up a reaction to digest DNA with restriction enzymes. The final volume will be 20 ?μl. How much of each of the following reagents will you use to obtain the indicated working concentrations? 10x restriction buffer >> working concentration 1x 1 mg/mL bovine serum albumin (BSA) >> working concentration 100?μg/mLWhat is the implication of the amount of nucleic acid in cells for diagnostic testing or forensic testing? Explain your answer
