A. Determine the concentration of the glucose stock solution in mg/mL. B. Complete the table above by answering items (a) to (o). Show one (1) representative calculation for each data (volume, dilution factor, and concentration).

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A. Determine the concentration of the glucose stock solution in mg/mL.
B. Complete the table above by answering items (a) to (o). Show one (1)
representative calculation for each data (volume, dilution factor, and
concentration).
Transcribed Image Text:A. Determine the concentration of the glucose stock solution in mg/mL. B. Complete the table above by answering items (a) to (o). Show one (1) representative calculation for each data (volume, dilution factor, and concentration).
5) A stock solution of glucose was prepared by dissolving 500 mg of glucose in
distilled water to a final volume of 25 mL From this stock solution, standard
solutions were prepared by taking different volume of aliquots and diluting it
with distilled water to a final volume of 2.00 mL. Nelson's reagent was added to
the prepared samples (see table below).
Volume of
Volume of
Final volume
Concentration
Dilution
glucose stock distilled water
solution (mL)
of dilution
of glucose,
Factor
(mL)
(mL)
mg/mL
(a)
1.75
2.00
(f)
(k)
(b)
1.50
2.00
(g)
(1)
(c)
1.25
2.00
(h)
(m)
(d)
1.00
2.00
(0)
(n)
(e)
0.75
2.00
(o)
Transcribed Image Text:5) A stock solution of glucose was prepared by dissolving 500 mg of glucose in distilled water to a final volume of 25 mL From this stock solution, standard solutions were prepared by taking different volume of aliquots and diluting it with distilled water to a final volume of 2.00 mL. Nelson's reagent was added to the prepared samples (see table below). Volume of Volume of Final volume Concentration Dilution glucose stock distilled water solution (mL) of dilution of glucose, Factor (mL) (mL) mg/mL (a) 1.75 2.00 (f) (k) (b) 1.50 2.00 (g) (1) (c) 1.25 2.00 (h) (m) (d) 1.00 2.00 (0) (n) (e) 0.75 2.00 (o)
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