a. What does ECD mean. b. Discuss how column temperature influences separation in a chromatography experiment? c. Proportionality constant Fis known as

Q: two chromatograms after LC separation. Which chromatogram is better to proceed for MS analysis? What…

A: LCMS is Liquid chromatography–mass spectrometry used  for separation, identification and percentage…

Q: Why is a suppressor used in ion chromatography?

A: Ion chromatography- It is used for water chemistry analysis. Ion chromatography helps in measuring…

Q: How does the polarity of the mobile phase affect chromatograms?

A: Chromatograms are the pattern formed on an adsorbent medium by the layers of components separated by…

Q: Explain

A: In TLC analysis we put solvent below the sample spot .

Q: What are the properties that determine retention time in the chromatography process

A: ANSWER IS

Q: Metal ions chelate polar groups of analyte molecules for longer retention time. Does this affect…

A: HPLC is based on the principle where the sample (analyte) is distributed between a mobile phase…

Q: What would happen if we use longer columns and select adsorbing materials with 200-400 mesh sizes…

A: Solution is given in the following steps:

Q: In all forms of chromatography, there is a mobile phase and a stationary phase.  In thin layer…

A: Thin layer chromatography is the synthetic technique used in determining the purity of compound,…

Q: In Thin Layer Chromatography，why does the least polar of the analytes （biphenyl）have a higher Rf…

A:

Q: What is the role of the stationary phase and mobile phase in chromatography?

A: Solution:    Chromatography is a laboratory separating technique for the mixture. The mixture of…

Q: . What is lon chromatography?

A: chromatography  : Chromatography can be defined as the chromatography is a physical technique of…

Q: Cite problems why t

A:

Q: Give Four main precautions need to be considered when carrying out the following.

A: ANSWER IS :

Q: 2. In HPLC analysis, why Would peak area is preferred to peak height in evaluating the caffeine…

A: Since you have asked multiple questions, we will answer the first question for you. To get the…

Q: Chemistry Briefly describe the differences between the scanning and SIM approaches with reference…

A: Answer - According to the question - (TIC) - A TIC (Total Ion Chromatogram) is a chromatogram…

Q: What accounts for a high

A: Chromatography is a technique used for separation and identification of the components in mixture…

Q: Explain why a chromatographic separation normally has an optimum flow rate that gives the best…

A: When an fast flow rate is maintained, the band is not allowed to spend much time within the column…

Q: You are monitoring the reaction A --> B via periodic GC analysis. Initially, you only see one peak…

A: GC analysis is analytical technique by which separation of chemical mixture into its constituents…

Q: In thin-layer chromatography, what will be the result if the solvent level is higher than the sample…

A: A multiple choice question based on thin layer chromatography, which is to be accomplished.

Q: What is the objective of the Separation Technique: Paper Chromatography ?

A: A separation process is a method that converts a mixture or solution of chemical substances into two…

Q: how might chromatography be used to analyze the composition of the dye in a marker?

A: Chromatography is a method for analyzing mixtures by separating them into the chemicals from which…

Q: a pigment in the thin layer chromatography traveled beyond or farther than the solvent front.…

A: As a pigment in the thin layer chromatography traveled beyond or farther the solvent front therefore…

Q: SFC combines  important advantages of gas and liquid chromatography. It is especially useful for…

A: The chromatography is the separation technique used for identification and separation of compounds…

Q: [1] In paper chromatography, why do some ions move only a short distance from the point of…

A: In paper chromatography, the stationary phase is the water present in trapped state between the…

Q: Preparing a Thin Layer Chromatography experiment there are some common mistakes. Explain what…

A: Interpretation- To tell about all the common mistakes when we prepare a thin layer chromatography…

Q: Why does silanization reduce tailing of chromatographic peaks?

A: Given observation,  Silanization reduces tailing of chromatographic peaks.

Q: In normal phase chromatography, water is a weaker eluent than methanol., True or False?

A:

Q: How might the Rf values of colorless substances be determined? That is, how could the colorless…

A: we use locating agents which reacts with the substance to form colored compound

Q: In HPLC, why is purging or initialization being done before analysis of samples, explain.

A: ✓basically following two reasons for using purging and initialization during HPLC sample preparation…

Q: What properties should the stationary-phase liquid for gas chromatography possess?

A: Desirable properties of the stationary phase for GC include low volatility, thermal stability,…

Q: Separation of Peaks Below are two chromatograms after LC separation. Which chromatogram is better to…

A: LCMS is Liquid chromatography–mass spectrometry which is used for mass analysis of unknown or known…

Q: Why ink must never be used to mark the TLC (Thin-Layer Chromatography) plate?

A: Thin chromatography plate is very commonly used in industries for identifying of a compound . Purity…

Q: How can TLC be used to determine if a sample is pure?

A: Thin Layer Chromatography (TLC) is a separation procedure that requires a very small sample. It is…

Q: What is Jon chromatographys

A: Given To know about the chromatography

Q: by

A: Chromatography is basically that technique which allows the separation, identification, and…

Q: Answer in your own words. What is the advantage of very small particle sizes in Liquid solid…

A:

Q: How to determine which is the most polar and least polar in REVERSE PHASE thin-layer chromatography?…

A: Chromatography technique is used to separate compounds from mixture of compounds.Let's discuss the…

Q: 5. Column Chromatography works by the same principle as TLC however the silica gel is not plated on…

A: To answer question 5.

Q: What is the basic principle for Thin layer chromatography? How chromatogram is developed in thin…

A: ABOUT THIN LAYER CHROMATOGRAPHY :

Q: The following chromatographic peaks indicate the undesirable interaction and separation that taken…

A: Abnormal chromatographic peaks:

Q: what chromatography seperation how is tlc part of it

A:

Q: Why do brands broaden as they travel through a chromatography column ?

A: In analytical chemistry, To separate a single compound from a mixture, Column chromatography is…

Q: In thin layer chromatography what is the mobile phase?

A: Chromatography is a technique used for the separation of non-volatile liquids.

Q: Why are peak areas used instead of peak height in Reverse Phase HPLC of Caffeine and Benzoic Acid?

A: In case of carrying out HPCL of caffeine and benzoic acid, peak heights are better option for…

Q: Explain how to check the purity of compund using thin layer chromatography

A:

Q: c) In one paper chromatography, the Rf for spots X and Y are 0.5 and 0.35 respectively. The solvent…

A: Paper chromatography is the chromatography based on liquid-liquid partition chromatography. It…

Q: thin layer chromatography: What would happen to the Rf value of a compound if you increased the…

A: One of the methods of identification of the individual compounds from a mixture of compounds is TLC.…

Q: 4. Why is it so important that you don't erase mistakes on the chromatography раper?

A: Given : Chromatography paper To Find: Reason for not erasing mistakes on chromatography paper…