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- During proofreading, which of the following enzymes reads the DNA? primase topoisomerase DNA pol helicaseTranscribe and translate the following DNA sequence (nontemplate strand): 5'- ATGGCCGGTTATTAAGCA-3'WORKSHEET TASK 3: 1. Below is a theoretical section of DNA. Design two primers that are 10 base pairs (bp) long that will amplify this section of DNA in a PCR reaction (‘N’ refers to non-specific ‘nucleotide’). 3’–A C G T G A A C T G C C T NNN......NNN C C G T G T A T C T C T T–5’ 5’–T G C A C T T G A C G G A NNN......NNN G G C A C A T A G A G A A–3’
- 54. Which primer will anneal to this strand, and elongate during PCR? 5’ ATGCGGTTAC 3’ Group of answer choices 5’ GTAA 3’ 5’ ATGC 3’ 5’ TTAC 3’ 5’ GCAT 3’28.Bacteria X contains the double stranded DNA sequence below. Our task is to design a labelled primer (probe) to detect this sequence. Which sequence below can be used for this task? Identify the single most correct answer below. * indicates a label 5' ATGCTTTTTTTAAACCCCGGGGTTTT 3' 3'TACGAAAAAATTTGGGGCCCCAAAA 5' a) 3' AATTTGGGGC* ( b) 3' ATGCTTTTTT* ( ) c) 5' TTTAAAGGG* 19 ( ) d) 5' ATGCTTTTTT* ) e) a and b () f) a and d 23 g) b and d 29.Ames assay is patented, so 2nd yr microbiology students devised a new assay where wild type GFP (green florescence protein AKA light producing) is used as the target gene, for mutation rate analysis. When compound A is added, even in very low concentrations, all colonies formed end up producing light (produce fluoresce). Therefore, one can conclude that compound A is a strong mutagen. () True False 30.The consensus SD (Shine/Dalgarno sequence) is 5' AGGAGGU. Identify the single most correct answer below. a) The SD sequence 3' AGGAGGU is stronger than…I need help defining what is DNA Chromatography.
- Need help, please. Please answer the following four questions to the image I attached. 1. You digest both plasmids with BamHI and SacI together (a double digest). What size fragments will result? Please indicate the sizes of the expected fragments in basepairs: The large fragment of pNUT is fragment A. This fragment is expected to be ____ bp. 2. The small fragment of pNUT is fragment B. This fragment is expected to be ____ bp. 3. The large fragment of pPOD is fragment C. This fragment is expected to be ____ bp. 4. The small fragment of pPOD is fragment D. This fragment is expected to be ____ bp.options for each are: primase, topoisomerase, dnaB, dnaA, Pol IIICan you explain thie each of the statement given i dont really understand the dna recombinant is used as a molecular cloning and application for recombinant dna
- 28. What type of mutation is seen here? WT: 5′-AUG GCU AGA GUU GAA AAA-3′ Mutant: 5′-AUG GGU AGA GUU GAA AAA-3′ Group of answer choices 1. Tranversion 2. Deletion 3. Transition 4. InsertionResults obtained from a Nano Spec. DNA samples collected: ng/uL A260/A80 A260/A230 E.coli genomic DNA 45.3 1.68 0.84 pEMBL1.9 26.0 1.80 1.09 pBluescript 82.1 1.90 1.89 What does this results tell us about the purity of the DNA samples? What are possible contaminations that may have occured?The following are DNA fragments containing a small gene. The top strand is the coding strand. Transcribe all groups and translate. FIND THE POSSIBLE MUTATIONS Group D 5’-GGCAATGGGTTTGTGCAATTCTAACAGTTTTTAATTC-3’ 3’-CCGTTACCCAAACACGTTAAGATTGTCAAAAATTAAG-5’ Group E 5’-GGCAATGGGTTTTGCAATTCTAAAAGTTTTTAATTC-3’ 3’-CCGTTACCCAAAACGTTAAGATTTTCAAAAATTAAG