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Provide an explanation of the effects which are responsible for the three absorbances profile in relation to the height of the burner. Figure below shows the absorbance profile of the three elements.
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- The absorbance values at 250nm of 5 standard solutions, and sample solution of a drug are given below: Conc. (ug/ml) A 250 nm10 0.16820. 0.32930 0.50840. 0.66050 0.846Sample. 0.661Calculate the concentration of the sampleWhite light is directed to a reflection grating at an incident angle of 45 degrees. How many grooves per mm should the grating have to diffract first order 666 nm light at an angle of -10 degrees. Round your answer to the nearest 1 groove/mm.What absorbance corresponds to 1% transmittance? To 50% transmittance?
- Based on the data on the table, show the calibration curve and determine the concentration of the water sample with an absorbance of 0.121. Concentration (ppm) Absorbance 0.0000 0.0240 0.0200 0.0320 0.0400 0.0500 0.0600 0.0680 0.0800 0.0820 0.1000 0.0900 0.2000 0.1690 0.3000 0.2390Why should we use quartz cuvettes for measuring absorbance in the UV range and plastic cuvettes in the visible range?As pH changes, ho will absorbance change t the wavelength of the isosbestic point?